淫羊藿苷通过上调α-烯醇化酶表达促进成骨分化。
Icariin promotes osteogenic differentiation by upregulating alpha-enolase expression.
作者信息
Xie Dingbang, Xu Yunteng, Cai Wanping, Zhuo Junkuan, Zhu Zaishi, Zhang Haifeng, Zhang Yimin, Lan Xin, Yan Hui
机构信息
College of Integrative Medicine, Laboratory of Pathophysiology, Key Laboratory of Integrative Medicine on Chronic Diseases (Fujian Province University), Synthesized Laboratory of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, China.
Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, China.
出版信息
Biochem Biophys Rep. 2023 Apr 17;34:101471. doi: 10.1016/j.bbrep.2023.101471. eCollection 2023 Jul.
Osteogenic differentiation is a crucial biological process for maintaining bone remodelling. Aerobic glycolysis is the main source of energy for osteogenic differentiation. Alpha-enolase (Eno1), a glycolytic enzyme, is a therapeutic target for numerous diseases. Icariin, a principal active component of the traditional Chinese medicine , can stimulate osteogenic differentiation. Here, we aimed to determine if icariin promotes osteogenic differentiation via Eno1. Icariin (1 μM) significantly promoted osteogenic differentiation of MC3T3-E1 cells. Icariin upregulated Eno1 protein and gene expressions during osteogenic differentiation. Moreover, ENOblock, a specific inhibitor of Eno1, markedly inhibited icariin-induced osteogenic differentiation. Futhermore, western blot assay showed that Eno1 might mediate osteogenic differentiation through the BMP/Smad4 signalling pathway. Collectively, Eno1 could be a promising drug target for icariin to regulate osteogenic differentiation.
成骨分化是维持骨重塑的关键生物学过程。有氧糖酵解是成骨分化的主要能量来源。α-烯醇化酶（Eno1）作为一种糖酵解酶，是多种疾病的治疗靶点。淫羊藿苷作为中药的主要活性成分，能够刺激成骨分化。在此，我们旨在确定淫羊藿苷是否通过Eno1促进成骨分化。淫羊藿苷（1μM）显著促进了MC3T3-E1细胞的成骨分化。淫羊藿苷在成骨分化过程中上调了Eno1蛋白和基因表达。此外，Eno1的特异性抑制剂ENOblock显著抑制了淫羊藿苷诱导的成骨分化。此外，蛋白质印迹分析表明，Eno1可能通过BMP/Smad4信号通路介导成骨分化。总体而言，Eno1可能是淫羊藿苷调节成骨分化的一个有前景的药物靶点。
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