Department of Molecular Biology and Genetics, Erzurum Technical University, Erzurum, Türkiye.
EcoTech Biotechnology, Ata Teknokent, Erzurum, Türkiye.
Prep Biochem Biotechnol. 2023 Nov;53(10):1306-1312. doi: 10.1080/10826068.2023.2201930. Epub 2023 May 4.
Hydrophobins are amphipathic proteins with small molecular weights produced in filamentous fungi. These proteins are highly stable due to the disulfide bonds formed between the protected cysteine residues. They have great potential for usage in many different fields such as surface modifications, tissue engineering, and drug transport systems because hydrophobins are surfactants and soluble in harsh mediums. In this study, it was aimed to determine the hydrophobin proteins responsible for the hydrophobicity of the super-hydrophobic fungi isolates in the culture medium and to carry out the molecular characterization of the hydrophobin producer species. As a result of measuring surface hydrophobicity by determining the water contact angle, five different fungi with the highest hydrophobicity were classified as by classical and molecular (ITS and D1-D2 regions) methods. Also, protein extraction according to the recommended method for obtaining hydrophobins from spores of these species indicated that the isolates have similar protein profiles. Ultimately, the isolate named A5 with the highest water contact angle was identified as , and the 7 kDa band was appointed as a hydrophobin since it was the most abundant protein in protein extraction for this species.
水蛋白是一种由丝状真菌产生的、具有小分子量的两亲性蛋白。由于保护性半胱氨酸残基之间形成的二硫键，这些蛋白质具有高度的稳定性。由于水蛋白是表面活性剂，并且可溶于苛刻的介质中，因此它们在许多不同的领域都具有很大的应用潜力，如表面修饰、组织工程和药物传输系统。在这项研究中，旨在确定负责培养基中超级疏水性真菌分离物疏水性的水蛋白蛋白，并对水蛋白产生菌进行分子特征分析。通过测定水接触角来测量表面疏水性，根据经典和分子（ITS 和 D1-D2 区）方法，将 5 种疏水性最高的真菌分为 。此外，根据从这些 种的孢子中获得水蛋白的推荐方法进行蛋白提取，表明分离物具有相似的蛋白图谱。最终，水接触角最高的分离物 A5 被鉴定为 ，并且 7 kDa 条带被指定为水蛋白，因为它是该种蛋白提取中最丰富的蛋白。
