α-N-乙酰化β-内啡肽在正常及下丘脑-垂体分离（HPD）母羊中的加工过程存在差异。

alpha-N-acetylated beta-endorphin is differentially processed in the normal and hypothalamo-pituitary-disconnected (HPD) ewe.

作者信息

Smith A I, Wallace C A, Autelitano D J, Cheng M C, Clarke I J, Funder J W

出版信息

Neurosci Lett. 1986 Apr 11;65(2):229-33. doi: 10.1016/0304-3940(86)90310-1.

Abstract

We have used specific radioimmunoassay and high-performance liquid chromatography (HPLC) to determine content and molecular forms of pituitary alpha-N-acetylated endorphin (NacEP) in the intact and hypothalamo-pituitary-disconnected (HPD) sheep. No significant differences were seen in anterior pituitary (AP) immunoreactive (ir-) NacEP levels (control = 4.34 +/- 0.45 ng/mg; HPD = 4.38 +/- 0.83 ng/mg; n = 4) or in HPLC profiles following pituitary disconnection. In contrast, levels of ir-NacEP in the HPD intermediate lobe (IL) (263 +/- 16.5 ng/mg; n = 4) were double those in the control (110 +/- 14 ng/mg; n = 4), accompanied by a marked change in the molecular profile. In the control IL, the major species were Nac alpha EP (30%), Nac gamma EP (30%) and Nac beta EP1-27 (35%); in HPD IL, Nac beta EP1-27 (65%) is the dominant molecular form, with Nac alpha EP (11%) and Nac gamma EP (9%) relatively minor components.

摘要

我们使用特定的放射免疫分析和高效液相色谱法（HPLC）来测定完整和下丘脑 - 垂体分离（HPD）绵羊中垂体α - N - 乙酰化内啡肽（NacEP）的含量和分子形式。垂体前叶（AP）中免疫反应性（ir - ）NacEP水平（对照组 = 4.34±0.45 ng/mg；HPD组 = 4.38±0.83 ng/mg；n = 4）或垂体分离后的HPLC图谱均未观察到显著差异。相比之下，HPD中间叶（IL）中ir - NacEP水平（263±16.5 ng/mg；n = 4）是对照组（110±14 ng/mg；n = 4）的两倍，同时分子图谱发生了显著变化。在对照中间叶中，主要成分是NacαEP（30%）、NacγEP（30%）和NacβEP1 - 27（35%）；在HPD中间叶中，NacβEP1 - 27（65%）是主要分子形式，NacαEP（11%）和NacγEP（9%）是相对较少的成分。