一种用于测量细胞内外蛋白质的混合技术。

Department of Periodontology, Henry M. Goldman School of Dental Medicine, Boston University, Boston, MA, United States of America.

PLoS One. 2023 May 4;18(5):e0282948. doi: 10.1371/journal.pone.0282948. eCollection 2023.

ELISA or Western blot is known as a basic technique to be used for measurement of intracellular proteins, but in some cases, they cannot overcome problems such as normalization between samples or extraneous costs for required commercial kits. In order to address this problem, we developed a rapid and effective method (a hybrid of Western blot and ELISA). We use this new hybrid method to detect and normalize trace protein changes in gene expression intracellularly at a lower cost.

ELISA 或 Western blot 被认为是一种用于测量细胞内蛋白质的基本技术，但在某些情况下，它们无法克服样品间的标准化或所需商业试剂盒的额外成本等问题。为了解决这个问题，我们开发了一种快速有效的方法（Western blot 和 ELISA 的混合）。我们使用这种新的混合方法以更低的成本检测和标准化基因表达细胞内痕量蛋白质变化。