Chen To-Wen, Gandotra Rishabh, Chang Hwan-You, Lee Mel S, Kuo Feng-Chih, Lee Gwo-Bin
Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
Institute of NanoEngineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan.
Anal Chem. 2023 May 16;95(19):7693-7701. doi: 10.1021/acs.analchem.3c00767. Epub 2023 May 5.
Post-arthroplasty periprosthetic joint infection (PJI) is a serious ailment that can be difficult to diagnose. Herein, we developed a novel integrated microfluidic system (IMS) capable of detecting two common PJI biomarkers, alpha defensin human neutrophil peptide 1 (HNP-1) and C-reactive protein (CRP), from synovial fluid (SF). A magnetic bead-based one-aptamer-one-antibody assay was carried out automatically within 45 min on a single chip for simultaneous detection of both biomarkers at concentration ranges of 0.01-50 (HNP-1) and 1-100 (CRP) mg/L. It is the first report for utilizing these two biomarkers as targets to establish the new one-aptamer-one-antibody assay to detect PJI on-chip, and the aptamers demonstrated high specificity to their SF targets. As 20 clinical samples were correctly diagnosed with our IMS (verified by a common gold standard kit), it could serve as a promising tool for PJI diagnostics.
关节置换术后假体周围关节感染（PJI）是一种严重的疾病，可能难以诊断。在此，我们开发了一种新型集成微流控系统（IMS），能够从滑液（SF）中检测两种常见的PJI生物标志物，即α防御素人类中性粒细胞肽1（HNP-1）和C反应蛋白（CRP）。基于磁珠的一适体一抗体检测在单个芯片上45分钟内自动完成，可同时检测浓度范围为0.01 - 50（HNP-1）和1 - 100（CRP）mg/L的两种生物标志物。这是首次报道以这两种生物标志物为靶点建立新的一适体一抗体检测方法用于芯片上检测PJI，且适体对其滑液靶点显示出高特异性。由于我们的IMS正确诊断了20份临床样本（经通用金标准试剂盒验证），它可作为一种有前景的PJI诊断工具。
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