纳米孔测序技术在血流感染病原学诊断中的应用与展望

[Application and Prospect of Nanopore Sequencing Technology in Etiological Diagnosis of Blood Stream Infection].

作者信息

Guo Wei, Fan Shuai-Hua, DU Peng-Cheng, Guo Jun

机构信息

Department of Respiratory and Critical Care Medicine,Beijing Tsinghua Changgung Hospital Affiliated to Tsinghua University,School of Clinical Medicine,Tsinghua University,Beijing 102218,China.

Beijing Key Laboratory of Emerging Infectious Diseases,Institute of Infectious Diseases,Beijing Ditan Hospital Affiliated to Capital Medical University,Beijing 100015,China.

出版信息

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2023 Apr;45(2):317-321. doi: 10.3881/j.issn.1000-503X.14995.

DOI:10.3881/j.issn.1000-503X.14995

PMID:37157082

Abstract

Blood stream infection (BSI),a blood-borne disease caused by microorganisms such as bacteria,fungi,and viruses,can lead to bacteremia,sepsis,and infectious shock,posing a serious threat to human life and health.Identifying the pathogen is central to the precise treatment of BSI.Traditional blood culture is the gold standard for pathogen identification,while it has limitations in clinical practice due to the long time consumption,production of false negative results,etc.Nanopore sequencing,as a new generation of sequencing technology,can rapidly detect pathogens,drug resistance genes,and virulence genes for the optimization of clinical treatment.This paper reviews the current status of nanopore sequencing technology in the diagnosis of BSI.

摘要

血流感染（BSI）是一种由细菌、真菌和病毒等微生物引起的血液传播疾病，可导致菌血症、脓毒症和感染性休克，对人类生命和健康构成严重威胁。识别病原体是精确治疗BSI的关键。传统血培养是病原体鉴定的金标准，但由于耗时较长、会产生假阴性结果等原因，在临床实践中存在局限性。纳米孔测序作为新一代测序技术，能够快速检测病原体、耐药基因和毒力基因，以优化临床治疗。本文综述了纳米孔测序技术在BSI诊断中的现状。