牛对奥斯特他线虫第三期幼虫可溶性提取物的血清抗体反应。

Serum antibody response to soluble extract of the third-larval stage of Ostertagia ostertagi in cattle.

作者信息

Klesius P H, Washburn S M, Haynes T B

出版信息

Vet Parasitol. 1986 Apr;20(4):307-14. doi: 10.1016/0304-4017(86)90128-7.

Abstract

Serum IgG, IgM, and IgA antibody responses against L3 antigens of Ostertagia ostertagi were monitored by enzyme-linked immunosorbent assay (ELISA) after one, two or multiple sequential inoculations of this nematode in calves. Following the first infection, antibody levels did not change. After a second inoculation, IgG increased significantly (P less than 0.05) after 2 months. IgG was not significantly increased 1 month after challenge inoculation. IgM and IgA antibody levels did not change following the first or second inoculations of L3. IgG antibody levels rose only slightly following multiple sequential inoculations with infectious L3. Results indicate that calves with ostertagiasis have very weak serum antibody responses to L3, and these appear to be of little value in detection of the infection in these animals.

摘要

在对犊牛进行一次、两次或多次连续接种奥斯特他线虫后，通过酶联免疫吸附测定（ELISA）监测针对奥斯特他线虫L3抗原的血清IgG、IgM和IgA抗体反应。首次感染后，抗体水平未发生变化。第二次接种后，2个月时IgG显著升高（P小于0.05）。攻击接种1个月后，IgG未显著升高。L3首次或第二次接种后，IgM和IgA抗体水平未发生变化。多次连续接种感染性L3后，IgG抗体水平仅略有上升。结果表明，患有奥斯特他线虫病的犊牛对L3的血清抗体反应非常微弱，这些反应在检测这些动物的感染方面似乎价值不大。