bioRxiv. 2023 Jun 16:2023.04.24.538049. doi: 10.1101/2023.04.24.538049.
Pathological loss-of-function mutations in cyclin-dependent kinase-like 5 ( ) cause CDKL5 deficiency disorder (CDD), a rare and severe neurodevelopmental disorder associated with severe and medically refractory early-life epilepsy, motor, cognitive, visual and autonomic disturbances in the absence of any structural brain pathology. Analysis of genetic variants in CDD have indicated that CDKL5 kinase function is central to disease pathology. encodes a serine-threonine kinase with significant homology to GSK3b, which has also been linked to synaptic function. Further, knock-out rodents have increased GSK3b activity and often increased long-term potentiation (LTP). Thus, development of a specific CDKL5 inhibitor must be careful to exclude cross-talk with GSK3b activity. We synthesized and characterized specific, high-affinity inhibitors of CDKL5 that do not have detectable activity for GSK3b. These compounds are very soluble in water but blood-brain barrier penetration is low. In rat hippocampal brain slices, acute inhibition of CDKL5 selectively reduces post-synaptic function of AMPA-type glutamate receptors in a dose-dependent manner. Acute inhibition of CDKL5 reduces hippocampal LTP. These studies provide new tools and insights into the role of CDKL5 as a newly appreciated, key kinase necessary for synaptic plasticity. Comparisons to rodent knock-out studies suggest that compensatory changes have limited the understanding of the roles of CDKL5 in synaptic physiology, plasticity and human neuropathology.
细胞周期蛋白依赖性激酶样 5 ( )中的病理性失活突变导致 CDKL5 缺乏症 (CDD),这是一种罕见且严重的神经发育障碍,与严重且药物难治性的婴儿期癫痫、运动、认知、视觉和自主神经障碍有关,而没有任何结构脑病理学。对 CDD 中遗传变异的分析表明,CDKL5 激酶功能是疾病发病机制的核心。 编码一种丝氨酸-苏氨酸激酶,与 GSK3b 具有显著同源性,GSK3b 也与突触功能有关。此外, 敲除啮齿动物的 GSK3b 活性增加,并且通常长时程增强 (LTP) 增加。因此,开发特定的 CDKL5 抑制剂必须小心排除与 GSK3b 活性的交叉对话。我们合成并表征了对 GSK3b 没有可检测活性的特异性、高亲和力的 CDKL5 抑制剂。这些化合物在水中非常可溶,但血脑屏障渗透性低。在大鼠海马脑片上,CDKL5 的急性抑制以剂量依赖性方式选择性地降低 AMPA 型谷氨酸受体的突触后功能。CDKL5 的急性抑制降低海马 LTP。这些研究为 CDKL5 作为一种新发现的、对突触可塑性至关重要的关键激酶提供了新的工具和见解。与啮齿动物敲除研究的比较表明,代偿性变化限制了对 CDKL5 在突触生理学、可塑性和人类神经病理学中的作用的理解。