Iyoda I, Takahashi H, Lee L C, Okajima H, Inoue A, Sasaki S, Takeda K, Yoshimura M, Ijichi H
Cardiovasc Res. 1986 Apr;20(4):294-8. doi: 10.1093/cvr/20.4.294.
To investigate the effects of sodium-potassium activated adenosine triphosphatase inhibition on central cardiovascular regulation a microinjection of ouabain was given into the hypothalamus of urethane anaesthetised rats. Doses of 0.01-1.0 micrograms per rat injected into the posterior hypothalamus produced a rise in blood pressure within 1 min, the maximum rise occurring 15-20 min later in a dose dependent manner. Both heart rate and abdominal sympathetic nerve activity increased with the rise in blood pressure. Ouabain injected into either the anterior preoptic hypothalamus or the ventromedial hypothalamus produced no notable cardiovascular responses. These results suggest that an endogenous digitalis like substance produced in the hypothalamus as a result of sodium loading may participate in central cardiovascular regulation by increasing sympathetic outflow in the discrete area of the brain, as does ouabain.
为研究钠钾激活的三磷酸腺苷酶抑制对中枢心血管调节的影响，将哇巴因微量注射到乌拉坦麻醉大鼠的下丘脑。向后下丘脑注射每只大鼠0.01 - 1.0微克的剂量，1分钟内血压升高，最大升高在15 - 20分钟后出现，呈剂量依赖性。随着血压升高，心率和腹部交感神经活动均增加。向前视前下丘脑或腹内侧下丘脑注射哇巴因未产生明显的心血管反应。这些结果表明，钠负荷导致下丘脑产生的一种内源性洋地黄样物质可能像哇巴因一样，通过增加大脑离散区域的交感神经输出参与中枢心血管调节。
