Chang D C, Tasaki I
Cell Mol Neurobiol. 1986 Mar;6(1):43-53. doi: 10.1007/BF00742975.
The structure of the axolemma of the squid giant axon was studied by freeze-fracture electron microscopy. Three types of preparations were examined: intact axons, axons with their Schwann cell sheaths stripped off prior to freezing, and axons with their Schwann cell sheaths chemically detached but not mechanically removed. Because of a problem of cross-fracturing, the first two types of preparations revealed very few membrane faces of the axolemma. This cross-fracturing problem, however, was eliminated when we used a complementary replication method to fracture the third type of preparation. We found that the E-face of the axon membrane was smooth relative to the P-face, which showed many prominent intramembrane particles (IMP). The diameters of the typical IMP range from 6 to 15 nm. The P-face of the adjacent Schwann cells also showed many large IMP. The sizes and heights of the Schwann-cell IMP, however, appear to be more homogeneous than the P-face axolemma.
通过冷冻蚀刻电子显微镜研究了鱿鱼巨轴突轴膜的结构。检查了三种类型的标本：完整的轴突、在冷冻前剥去雪旺细胞鞘的轴突以及雪旺细胞鞘通过化学方法分离但未机械去除的轴突。由于交叉断裂问题，前两种类型的标本显示出很少的轴膜膜面。然而，当我们使用互补复制方法对第三种类型的标本进行断裂时，这个交叉断裂问题得到了解决。我们发现轴突膜的E面相对于P面是光滑的，P面显示出许多突出的膜内颗粒（IMP）。典型IMP的直径范围为6至15纳米。相邻雪旺细胞的P面也显示出许多大的IMP。然而，雪旺细胞IMP的大小和高度似乎比轴膜P面更均匀。
