Soil and Environmental Microbiology Laboratory, Department of Microbiology, Assam University, Silchar 788011, Assam, India.
Department of Biotechnology, AKS University, Satna 485001, Madhya Pradesh, India.
Bioresour Technol. 2023 Aug;382:129198. doi: 10.1016/j.biortech.2023.129198. Epub 2023 May 16.
Asphaltene is the most recalcitrant compound in crude oil. Bacteria were isolated from crude oil contaminated soil and their efficiency for hydrocarbon degradation was determined using GC-MS and isolates were screened for biosurfactant production using FT-IR. Two Bacillus spp. having hydrocarbonoclastic and lipo-peptide biosurfactant-producing abilities were experimented for their asphaltene removal potential through oil removal efficiency (ORE%) and asphaltene degradation efficiency (ADE%). B. thuringeinsis SSL1 and B. cereus SSL3 could degrade 76.4% and 67.4% of asphaltene (20 g L), in vitro, respectively, which is much higher than previous reports. B. thuringiensis SSL1 is recommended for effective breakdown of asphaltene, total petroleum hydrocarbon, and polyaromatic hydrocarbon degradation, aided by its biosurfactants, which is useful for crude oil cleanup. Biosurfactants are important for enhancing the availability of hydrophobic hydrocarbons to bacteria, which is beneficial for efficient crude oil remediation. These findings could lead to more effective strategies for complete clean-up of crude oil pollution.
沥青质是原油中最难降解的化合物。从受原油污染的土壤中分离出细菌，并利用 GC-MS 测定其烃类降解效率，同时利用 FT-IR 筛选具有生物表面活性物质生产能力的分离物。通过油去除效率（ORE%）和沥青质降解效率（ADE%），实验了两种具有烃类分解和脂肽生物表面活性剂生产能力的芽孢杆菌属（Bacillus spp.），以研究其去除沥青质的潜力。B. thuringeinsis SSL1 和 B. cereus SSL3 分别可以在体外降解 20 g/L 沥青质的 76.4%和 67.4%，这比以前的报道要高得多。B. thuringiensis SSL1 被推荐用于有效分解沥青质、总石油烃和多环芳烃降解，这得益于其生物表面活性剂，这对于原油清理非常有用。生物表面活性剂对于提高疏水性碳氢化合物对细菌的可利用性很重要，这有利于高效的原油修复。这些发现可能会为彻底清除原油污染提供更有效的策略。
