PM2.5 通过降低细胞内 NAD 水平介导小鼠睾丸 SertoliTM4 细胞损伤。

PM2.5 mediates mouse testis Sertoli TM4 cell damage by reducing cellular NAD.

机构信息

Department of Infectious Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, P.R. China.

Department of Nosocomial Infection Management, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, P.R. China.

出版信息

Toxicol Mech Methods. 2023 Oct;33(8):636-645. doi: 10.1080/15376516.2023.2215862. Epub 2023 Jun 1.

DOI:10.1080/15376516.2023.2215862

PMID:37202861

Abstract

OBJECTIVE

This study aims to explore the mechanism of PM2.5 damage to the reproductive system of male mice.

METHODS

Mouse testis Sertoli TM4 cells were divided into four groups: a control group (no additional ingredients except for medium), PM2.5 group (medium containing 100 μg/mL PM2.5), PM2.5 + NAM group (medium containing 100 μg/mL PM2.5 and 5 mM NAM), and NAM group (medium containing 5 mM nicotinamide) and cultured for 24 or 48 h. The apoptosis rate of TM4 cells was measured using flow cytometry, the intracellular levels of NAD and NADH were detected using an NAD/NADH assay kit, and the protein expression levels of SIRT1 and PARP1 were determined by western blotting.

RESULTS

Mouse testis Sertoli TM4 cells exposed to PM2.5 demonstrated an increase in the apoptosis rate and PARP1 protein expression, albeit a decrease in NAD, NADH, and SIRT1 protein levels ( = 0.05). These changes were reversed in the group treated with a combination of PM2.5 and nicotinamide ( = 0.05).

CONCLUSION

PM2.5 can cause Sertoli TM4 cell damage in mouse testes by decreasing intracellular NAD levels.

摘要

目的

本研究旨在探讨 PM2.5 对雄性小鼠生殖系统损伤的作用机制。

方法

将小鼠睾丸 Sertoli TM4 细胞分为四组：对照组（除培养基外无其他添加物）、PM2.5 组（含 100μg/mL PM2.5 的培养基）、PM2.5+NAM 组（含 100μg/mL PM2.5 和 5mM NAM 的培养基）和 NAM 组（含 5mM 烟酰胺的培养基），培养 24 或 48 小时。采用流式细胞术检测 TM4 细胞的凋亡率，用 NAD/NADH 检测试剂盒检测细胞内 NAD 和 NADH 水平，用 Western blot 检测 SIRT1 和 PARP1 蛋白的表达水平。