维生素 D 补充对绝经后妇女热休克蛋白的调节作用：一项随机、双盲、安慰剂对照研究。

Impact of vitamin D supplementation on modulating heat-shock proteins in postmenopausal women: a randomized, double-blind, placebo-controlled study.

机构信息

Applied Physiology, Nutrition and Exercise Research Group (PhyNEr), Institute of Health Sciences, Federal University of Triangulo Mineiro (UFTM), Uberaba, Brazil.

From the Department Health Science, Oeste Paulista University-UNOESTE, Jaú, Brazil.

出版信息

Menopause. 2023 Jul 1;30(7):758-765. doi: 10.1097/GME.0000000000002197.

DOI:10.1097/GME.0000000000002197

PMID:37220771

Abstract

OBJECTIVE

The aim of this study was to examine the effect of vitamin D (VitD) supplementation on serum heat-shock proteins (HSP) in postmenopausal women (PW).

METHODS

In this double-blind, placebo-controlled trial, 160 PW aged 45 to 65 years with amenorrhea 12 months or more were randomized into two groups: 80 PW in VitD group (oral supplementation with 1,000 IU VitD 3 /d) or 80 PW in placebo group. The PW were assessed at baseline and after 10 months of intervention. Plasma concentrations of 25-hydroxyVitD (25[OH]D) were measured by high-performance liquid chromatography. HSP27/pS78/pS82, HSP27/total, HSP60, HSP70/72, and HSP90α levels were assessed in serum using a multiplexed bead immunoassay.

RESULTS

HSP27 (pS78/pS82 [ P < 0.035] and total [ P < 0.001]) levels increased in the supplemented group when compared with the control group. There was no effect of VitD supplementation on HSP60, HSP70/72, and HSP90α levels.

CONCLUSIONS

Vitamin D supplementation increases serum HSP27 level in PW.

摘要

目的

本研究旨在探讨维生素 D（VitD）补充对绝经后妇女（PW）血清热休克蛋白（HSP）的影响。

方法

在这项双盲、安慰剂对照试验中，将 160 名年龄在 45 至 65 岁之间、绝经 12 个月或以上的 PW 随机分为两组：80 名 PW 进入 VitD 组（口服补充 1000IU VitD 3 /d），80 名 PW 进入安慰剂组。PW 在基线和干预 10 个月后进行评估。采用高效液相色谱法测定血浆 25-羟维生素 D（25[OH]D）浓度。采用多重珠免疫分析测定血清 HSP27/pS78/pS82、HSP27/总、HSP60、HSP70/72 和 HSP90α 水平。