Guo X Q, Zhang S, Zheng H, Wang F, Miao N, Su Q D, Bi S L, Zhang G M, Wang F Z, Shen L P
National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Department of National Immunization Program, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2023 May 10;44(5):759-764. doi: 10.3760/cma.j.cn112338-20221130-01021.
To understand the distribution of genotypes and sub-genotypes of HBV in different ethnic groups in China. The HBsAg positive samples were selected by stratified multi-stage cluster sampling from the sample base of national HBV sero-epidemiological survey in 2020 for the amplification of S gene of HBV by nested PCR. A phylogeny tree was constructed to determine the genotypes and sub-genotypes of HBV. The distribution of genotypes and sub-genotypes of HBV were analyzed comprehensively by using laboratory data and demographic data. A total of 1 539 positive samples from 15 ethnic groups were successfully amplified and analyzed, and 5 genotypes (B, C, D, I and C/D) were detected. The proportion of genotype B was higher in ethnic group of Han (74.52%, 623/836), Zhuang (49.28%, 34/69), Yi (53.19%, 25/47), Miao (94.12%, 32/34), Buyi (81.48%, 22/27). The proportions of genotype C were higher in ethnic groups of Yao (70.91%, 39/55). Genotype D was the predominant genotype in Uygur (83.78%, 31/37). Genotype C/D were detected in Tibetan (92.35%,326/353). In this study, 11 cases of genotype I were detected, 8 of which were distributed in Zhuang nationality. Except for Tibetan, sub-genotype B2 accounted for more than 80.00% in genotype B in all ethnic groups. The proportions of sub-genotype C2 were higher in 8 ethnic groups, i.e. Han, Tibetan, Yi, Uygur, Mongolian, Manchu, Hui and Miao. The proportions of sub-genotype C5 were higher in ethnic groups of Zhuang (55.56%, 15/27) and Yao (84.62%, 33/39). For genotype D, sub-genotype D3 was detected in Yi ethnic group and sub-genotype D1 was detected in both Uygur and Kazak. The proportions of sub-genotype C/D1 and C/D2 in Tibetan were 43.06% (152/353) and 49.29% (174/353). For all the 11 cases of genotype I infection, only sub-genotype I1 was detected. Five genotypes and 15 sub-genotypes of HBV were found in 15 ethnic groups. There were significant differences in the distribution of genotypes and sub-genotypes of HBV among different ethnic groups.
为了解中国不同民族中乙型肝炎病毒（HBV）基因型和亚基因型的分布情况。通过分层多阶段整群抽样，从2020年全国HBV血清流行病学调查样本库中选取HBsAg阳性样本，采用巢式PCR扩增HBV的S基因。构建系统发育树以确定HBV的基因型和亚基因型。综合利用实验室数据和人口统计学数据，对HBV基因型和亚基因型的分布进行分析。共成功扩增并分析了来自15个民族的1539份阳性样本，检测到5种基因型（B、C、D、I和C/D）。B基因型在汉族（74.52%，623/836）、壮族（49.28%，34/69）、彝族（53.19%，25/47）、苗族（94.12%，32/34）、布依族（81.48%，22/27）中所占比例较高。C基因型在瑶族（70.91%，39/55）中所占比例较高。D基因型是维吾尔族的主要基因型（83.78%，31/37）。在藏族中检测到C/D基因型（92.35%，326/353）。本研究中检测到11例I基因型，其中8例分布在壮族。除藏族外，所有民族中B基因型中亚基因型B2占比均超过80.00%。C2亚基因型在汉族、藏族、彝族、维吾尔族、蒙古族、满族、回族和苗族这8个民族中所占比例较高。C5亚基因型在壮族（55.56%，15/27）和瑶族（84.62%，33/39）中所占比例较高。对于D基因型，在彝族中检测到D3亚基因型，在维吾尔族和哈萨克族中均检测到D1亚基因型。藏族中C/D1和C/D2亚基因型的比例分别为43.06%（152/353）和49.29%（174/353）。在所有11例I基因型感染病例中，仅检测到I1亚基因型。在15个民族中发现了5种HBV基因型和15种亚基因型。不同民族间HBV基因型和亚基因型的分布存在显著差异。
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