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综合转录组分析确定了水稻对氮和磷缺乏的协同反应。

Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice.

作者信息

Wang Fei, Wang Yan, Ying Luying, Lu Hong, Liu Yijian, Liu Yu, Xu Jiming, Wu Yunrong, Mo Xiaorong, Wu Zhongchang, Mao Chuanzao

机构信息

State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China.

Hainan Institute, Zhejiang University, Yazhou Bay Science and Technology City, Sanya, Hainan, China.

出版信息

Front Plant Sci. 2023 May 8;14:1164441. doi: 10.3389/fpls.2023.1164441. eCollection 2023.

Abstract

Nitrogen (N) and phosphorus (P) are two primary components of fertilizers for crop production. Coordinated acquisition and utilization of N and P are crucial for plants to achieve nutrient balance and optimal growth in a changing rhizospheric nutrient environment. However, little is known about how N and P signaling pathways are integrated. We performed transcriptomic analyses and physiological experiments to explore gene expression profiles and physiological homeostasis in the response of rice () to N and P deficiency. We revealed that N and P shortage inhibit rice growth and uptake of other nutrients. Gene Ontology (GO) analysis of differentially expressed genes (DEGs) suggested that N and Pi deficiency stimulate specific different physiological reactions and also some same physiological processes in rice. We established the transcriptional regulatory network between N and P signaling pathways based on all DEGs. We determined that the transcript levels of 763 core genes changed under both N or P starvation conditions. Among these core genes, we focused on the transcription factor gene () and show that its encoded protein is a positive regulator of P homeostasis and a negative regulator of N acquisition in rice. NIGT1 promoted Pi uptake but inhibited N absorption, induced the expression of Pi responsive genes and and repressed the N responsive genes and . These results provide new clues about the mechanisms underlying the interaction between plant N and P starvation responses.

摘要

氮(N)和磷(P)是作物生产肥料的两个主要成分。在不断变化的根际养分环境中,氮和磷的协同获取与利用对于植物实现养分平衡和最佳生长至关重要。然而,关于氮和磷信号通路如何整合的了解却很少。我们进行了转录组分析和生理实验,以探究水稻()对氮和磷缺乏的响应中的基因表达谱和生理稳态。我们发现氮和磷短缺会抑制水稻生长以及对其他养分的吸收。对差异表达基因(DEGs)的基因本体论(GO)分析表明,氮和磷缺乏会刺激水稻中特定的不同生理反应以及一些相同的生理过程。我们基于所有差异表达基因建立了氮和磷信号通路之间的转录调控网络。我们确定在氮或磷饥饿条件下,763个核心基因的转录水平发生了变化。在这些核心基因中,我们聚焦于转录因子基因(),并表明其编码蛋白是水稻中磷稳态的正调控因子和氮获取的负调控因子。NIGT1促进磷的吸收但抑制氮的吸收,诱导磷响应基因和的表达,并抑制氮响应基因和的表达。这些结果为植物氮和磷饥饿响应之间相互作用的潜在机制提供了新线索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ce/10200874/205b0176fc3a/fpls-14-1164441-g001.jpg

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