Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
Adv Exp Med Biol. 2023;1417:133-139. doi: 10.1007/978-981-99-1304-6_9.
Despite the advances in hepatitis E virus (HEV) cell infection models' development, HEV infection efficacy in these cell models is still low, which hampers the further study of molecular mechanism of HEV infection and replication and even the interaction between HEV and host. Along with the advances in the technology for liver organoids generation, major efforts will be made to develop liver organoids for HEV infection. Here, we summarize the entire new and impressive cell culture system of liver organoids and discuss their potential application in HEV infection and pathogenesis. Liver organoids can be generated from tissue-resident cells isolated from biopsies of adult tissues or from iPSCs/ESCs differentiation, which can expand the large-scale experiments such as antiviral drug screening. Different types of liver cells working together can recapitulate the liver organ maintaining the physiological and biochemical microenvironments to support cell morphogenesis, migration, and response to viral infections. Efforts to optimize the protocols for liver organoids generation will speed up the research for HEV infection and pathogenesis and even the antiviral drug identification and evaluation.
尽管戊型肝炎病毒 (HEV) 细胞感染模型的发展取得了进展，但这些细胞模型中的 HEV 感染效率仍然较低，这阻碍了对 HEV 感染和复制分子机制的进一步研究，甚至阻碍了 HEV 与宿主之间的相互作用。随着肝类器官生成技术的进步，将主要努力开发用于 HEV 感染的肝类器官。在这里，我们总结了整个新的令人印象深刻的肝类器官细胞培养系统，并讨论了它们在 HEV 感染和发病机制中的潜在应用。肝类器官可以从成人组织活检中分离的组织驻留细胞或从 iPSCs/ESCs 分化中生成，这可以扩展抗病毒药物筛选等大规模实验。不同类型的肝细胞共同作用，可以重现维持生理和生化微环境的肝脏器官，以支持细胞形态发生、迁移和对病毒感染的反应。优化肝类器官生成方案的努力将加速对 HEV 感染和发病机制的研究，甚至加速抗病毒药物的鉴定和评估。
