Ann Ital Chir. 2023;94:179-187.
Several studies have been conducted for the prevention of neuroma and recently published experimental studies include interventions on epineurium. The techniques which include interventions on epinerium were compared to reveal the role of epinurium in neuroma prevention.
55 Sprague-Dawley rats were divided into five groups. Two of the groups were negative and positive controls. The proximal nerve stump was left "free" in the negative control group, while the stump was implanted in a muscle pocket in the positive control group following sciatic nerve transection. Experimental groups include epineural ligation, epineural stripping and epineural capping procedures. Follow-up period was six months. After sacrification of the rats, histopathologic and immunohistochemical examinations were conducted as well as real-time PCR studies for the assessment. Statistical analysis was performed.
The most prominent neuroma formation was detected in the epineural capping group, while the least neuroma was observed in the epineural ligation group.
Statistically significant differences were obtained when the three experimental groups were compared with both control groups. Interestingly there was no significant difference in-between the control groups in terms of preventing neuroma formation.
epineural ligation group were found to be superior to both control groups as well as experimental groups. Use of epineural capping was concluded to increase the formation of neuroma rather than preventing. Intramuscular implantation of nerve stump had no preventive effect on neuroma formation.
Capping, Epineurium, Ligation, Neuroma, Stripping.
已经进行了多项预防神经瘤的研究，最近发表的实验研究包括对神经外膜的干预。将包括对外膜干预的技术与其他技术进行比较，以揭示神经外膜在预防神经瘤中的作用。
将 55 只斯普拉格-道利大鼠分为五组。其中两组为阴性和阳性对照组。在阴性对照组中，近端神经残端“自由”保留，而在阳性对照组中，坐骨神经切断后，残端植入肌肉袋中。实验组包括神经外膜结扎、神经外膜剥离和神经外膜套扎术。随访期为 6 个月。在处死大鼠后，进行组织病理学和免疫组织化学检查以及实时 PCR 研究评估。进行了统计分析。
在外膜套扎组中检测到最明显的神经瘤形成，而在外膜结扎组中观察到最少的神经瘤。
当将三个实验组与两个对照组进行比较时，得到了统计学上显著的差异。有趣的是，两组对照组在预防神经瘤形成方面没有显著差异。
神经外膜结扎组优于两个对照组和实验组。神经外膜套扎的使用被认为会增加神经瘤的形成，而不是预防。神经残端的肌内植入对神经瘤形成没有预防作用。
套扎、神经外膜、结扎、神经瘤、剥离。
