Plaschke M, Wenzel J, Dörner G, Tönjes R
J Hirnforsch. 1986;27(2):145-58.
During the first two weeks of life newborn male Wistar rats were subjected to social and nutritional deprivation. One group of the deprived animals was treated by daily injections of pyridostigmine (1 microgram/0.05 ml saline from Day 1 to Day 4, 5 micrograms/0.05 ml saline from Day 5 to Day 14). At the age of 14 days or 6 months, 5 deprived, 5 deprived and pyridostigmine treated animals and 5 controls were sacrificed by perfusion. The stratum radiatum of the hippocampal CA1-region was investigated for quantitative ultrastructural changes using electron microscopic, morphometric and stereologic methods. The following results were obtained: The number of synapses per unit area or per unit volume brain tissue (hippocampus) was found to be reduced by about 10% in 14 days old deprived as well as in deprived and pyridostigmine treated rats in comparison to the controls. At the age of 6 months both groups of the experimental animals showed an increase in the density of synapses by about 20% when compared to the controls. The proportion of axo-spinodendritic synapses in the total number of synapses (axo-dendritic and axo-spinodendritic synapses) was decreased on Day 14 and was increased at the age of 6 months in the neonatally deprived rats. Following additional pyridostigmine treatment, both in the juvenile and in the adult rats the ratio of axo-spinodendritic to axo-dendritic synapses was approximated to that of the controls. The calculation of the volume of all presynaptic terminals or all postsynaptic spines per unit volume brain tissue (volume density) revealed a decrease by about 6% (terminals) or 5% (spines) on Day 14 after deprivation and an increase by about 8% (terminals) or 14% (spines) following simultaneous pyridostigmine administration. At the age of 6 months the presynaptic terminals in early postnatally deprived rats showed a higher volume density by about 14%. In the deprived and pyridostigmine treated animals volume density enhancements were obtained for the terminals (by about 7%) and for the spines (by about 25%). The volume and surface of an individual presynaptic terminal were unchanged in 14 days old as well as in 6 months old deprived rats in comparison to the controls. Deprivation and simultaneous pyridostigmine treatment led to an increase in the volume (by about 25%) and the surface (by about 12%) of the terminals on Day 14. However, at the age of 6 months the volume and surface were reduced by about 14% or 12% when compared to the controls.(ABSTRACT TRUNCATED AT 400 WORDS)
在出生后的头两周，新生雄性Wistar大鼠遭受社会和营养剥夺。一组被剥夺的动物每天注射吡啶斯的明进行治疗（第1天至第4天，1微克/0.05毫升生理盐水；第5天至第14天，5微克/0.05毫升生理盐水）。在14天龄或6个月龄时，对5只被剥夺、5只被剥夺并经吡啶斯的明治疗的动物以及5只对照动物进行灌注处死。使用电子显微镜、形态计量学和体视学方法研究海马CA1区的辐射层，以进行定量超微结构变化分析。得到以下结果：与对照组相比，14天龄被剥夺以及被剥夺并经吡啶斯的明治疗的大鼠，每单位面积或每单位体积脑组织（海马）中的突触数量减少约10%。在6个月龄时，与对照组相比，两组实验动物的突触密度均增加约20%。在新生期被剥夺的大鼠中，轴突-棘状树突突触在突触总数（轴突-树突突触和轴突-棘状树突突触）中的比例在第14天降低，在6个月龄时增加。额外给予吡啶斯的明治疗后，无论是幼年大鼠还是成年大鼠，轴突-棘状树突突触与轴突-树突突触的比例均接近对照组。计算每单位体积脑组织中所有突触前终末或所有突触后棘的体积（体积密度）发现，剥夺后第14天，突触前终末体积密度降低约6%，突触后棘降低约5%；同时给予吡啶斯的明后，突触前终末体积密度增加约8%，突触后棘增加约14%。在6个月龄时，出生后早期被剥夺的大鼠的突触前终末体积密度高约14%。在被剥夺并经吡啶斯的明治疗的动物中，突触前终末和突触后棘的体积密度分别增加约7%和25%。与对照组相比，14天龄和6个月龄被剥夺的大鼠单个突触前终末的体积和表面积未发生变化。在第14天，剥夺并同时给予吡啶斯的明治疗导致突触前终末体积增加约25%，表面积增加约12%。然而，在6个月龄时，与对照组相比，体积和表面积分别减少约14%或12%。（摘要截选至400字）
