Truby P R
J Embryol Exp Morphol. 1986 Mar;92:115-31.
When the anteroposterior axis of a cockroach leg is reversed at a graft by exchanging a left leg for a right leg at the mid-tibia level, regeneration occurs in the region of the graft/host junction. This results in the formation of a pair of lateral supernumerary legs. In these experiments the patterns of cell division which take place during supernumerary leg formation were observed in sections of regenerating legs of the cockroach Leucophaea maderae. Early patterns of cell division resemble those seen in control grafts in which no axial reversal had been carried out during grafting. These cell divisions are associated with the process of wound healing. Later, a large area of the epidermis proximal to the graft/host junction becomes activated and shows a rapid rate of cell division. This area forms two outgrowths which grow by cell division throughout their epidermis to form the epidermis of the supernumerary legs. The results are more consistent with the view that the formation of supernumerary legs involves dedifferentiation of the epidermis in the region of the graft/host junction to form a blastema, rather than being due to local cell division at the point of maximum pattern discontinuity. This conclusion is used to offer an explanation for the range of different types of outcome of left-right grafts that has been observed.
当蟑螂腿的前后轴在移植时通过在胫节中部将左腿与右腿交换而反转时，移植/宿主交界处会发生再生。这导致形成一对外侧多余的腿。在这些实验中，在马德拉蜚蠊再生腿的切片中观察了多余腿形成过程中发生的细胞分裂模式。早期的细胞分裂模式类似于对照移植中的模式，在对照移植中，移植过程中未进行轴向反转。这些细胞分裂与伤口愈合过程相关。后来，移植/宿主交界处近端的一大片表皮被激活，并显示出快速的细胞分裂速率。该区域形成两个突起，通过整个表皮的细胞分裂生长，形成多余腿的表皮。结果更符合这样的观点，即多余腿的形成涉及移植/宿主交界处表皮的去分化以形成芽基，而不是由于最大模式不连续点处的局部细胞分裂。这一结论被用于解释所观察到的左右移植不同类型结果的范围。
