Yang Jing, Wang Shuren, Zhang Yundi, Chen Yunze, Zhou Heying, Zhang Guocai
College of Forestry, Guizhou University, Huaxi District, Guiyang 550025, China.
Heilongjiang Province Key Laboratory of Forest Protection, School of Forest, Northeast Forestry University, Hexing Road 26, Xiangfang District, Harbin 150040, China.
J Fungi (Basel). 2023 May 12;9(5):564. doi: 10.3390/jof9050564.
Black spot needle blight is a serious conifer disease of var. occurring in Northeast China, which is usually caused by the plant pathogenic fungus . From the diseased pine needles collected in Honghuaerji, the strain YJ-3 was isolated and identified as the phytopathogen, and its culture characteristics were studied. Then, we generated a highly contiguous 48.36-Mbp genome assembly (N50 = 6.62 Mbp) of the strain YJ-3 by combining the PacBio RS II Single Molecule Real Time (SMRT) and Illumina HiSeq X Ten sequencing platforms. The results showed that a total of 13,667 protein-coding genes were predicted and annotated using multiple bioinformatics databases. The genome assembly and annotation resource reported here will be useful for the study of fungal infection mechanisms and pathogen-host interaction.
黑斑针叶枯病是发生在中国东北的一种严重的针叶树病害，通常由植物病原真菌引起。从红花尔基采集的患病松针中，分离出菌株YJ-3并鉴定为植物病原体，同时对其培养特性进行了研究。然后，我们结合PacBio RS II单分子实时（SMRT）和Illumina HiSeq X Ten测序平台，生成了菌株YJ-3高度连续的48.36-Mbp基因组组装（N50 = 6.62 Mbp）。结果表明，使用多个生物信息学数据库共预测和注释了13,667个蛋白质编码基因。本文报道的基因组组装和注释资源将有助于研究真菌感染机制和病原体-宿主相互作用。
