Świątek Łukasz, Wasilewska Inga, Boguszewska Anastazja, Grzegorczyk Agnieszka, Rezmer Jakub, Rajtar Barbara, Polz-Dacewicz Małgorzata, Sieniawska Elwira
Department of Virology with Viral Diagnostics Laboratory, Medical University of Lublin, Chodźki 1, 20-093 Lublin, Poland.
Student Research Group, Department of Virology with Viral Diagnostics Laboratory, Medical University of Lublin, Chodźki 1, 20-093 Lublin, Poland.
Pharmaceutics. 2023 May 22;15(5):1561. doi: 10.3390/pharmaceutics15051561.
L. is a widely distributed plant used as a traditional herbal medicine, but the knowledge of its biological properties still needs to be improved. Thus, the purpose of this presented research was to assess the phytochemical profile of extracts from aerial parts of . , commercially available in Poland and to study their anticancer potential and antimicrobial properties, including the antiviral, antibacterial, and antifungal effects. Additionally, the bioactivity of fractions obtained from the hexane and ethyl acetate extract was analyzed. The phytochemical analysis revealed the presence of organic and phenolic acids, hydrolysable tannins (gallo- and ellagitannins), and flavonoids. Significant anticancer activity was found for hexane extract (GrH) and ethyl acetate extract (GrEA) with an SI (selectivity index) between 2.02 and 4.39. GrH and GrEA inhibited the development of HHV-1-induced cytopathic effect (CPE) in virus-infected cells and decreased the viral load by 0.52 log and 1.42 log, respectively. Among the analyzed fractions, only those obtained from GrEA showed the ability to decrease the CPE and reduce the viral load. The extracts and fractions from showed a versatile effect on the panel of bacteria and fungi. The highest activity was observed for fraction GrEA4 against Gram-positive bacteria, including ATCC 10240 (MIC 8 μg/mL), ATCC 12228 (MIC 16 μg/mL), ATCC 43300 (MIC 125 μg/mL), ATCC 29212 (MIC 125 μg/mL), and ATCC 6633 (MIC 125 μg/mL). The observed antibacterial effect may justify the traditional use of to treat hard-to-heal wounds.
L.是一种广泛分布的植物，用作传统草药，但对其生物学特性的了解仍有待完善。因此，本研究的目的是评估在波兰市场上可买到的L.地上部分提取物的植物化学特征，并研究其抗癌潜力和抗菌特性，包括抗病毒、抗菌和抗真菌作用。此外，还分析了从己烷和乙酸乙酯提取物中获得的馏分的生物活性。植物化学分析表明存在有机酸和酚酸、可水解单宁（没食子单宁和鞣花单宁）以及黄酮类化合物。发现己烷提取物（GrH）和乙酸乙酯提取物（GrEA）具有显著的抗癌活性，选择性指数（SI）在2.02至4.39之间。GrH和GrEA抑制病毒感染细胞中HHV - 1诱导的细胞病变效应（CPE）的发展，病毒载量分别降低0.52 log和1.42 log。在分析的馏分中，只有从GrEA获得的馏分显示出降低CPE和减少病毒载量的能力。L.的提取物和馏分对一系列细菌和真菌具有多种作用。观察到馏分GrEA4对革兰氏阳性菌的活性最高，包括金黄色葡萄球菌ATCC 10240（最低抑菌浓度8 μg/mL）、表皮葡萄球菌ATCC 12228（最低抑菌浓度16 μg/mL）、粪肠球菌ATCC 43300（最低抑菌浓度125 μg/mL）、肺炎链球菌ATCC 29212（最低抑菌浓度125 μg/mL）和酿脓链球菌ATCC 6633（最低抑菌浓度125 μg/mL）。观察到的抗菌作用可能解释了L.在治疗难愈合伤口方面的传统用途。
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