FKBP11 上调促进肝癌的增殖和迁移。

FKBP11 upregulation promotes proliferation and migration in hepatocellular carcinoma.

机构信息

Program and Department of Clinical Laboratory Medicine, Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.

Department of Medical Laboratory Science, Faculty of Medicine, Mbarara University of Science and Technology, Mbarara, Uganda.

出版信息

Cancer Biomark. 2023;37(2):109-120. doi: 10.3233/CBM-220440.

DOI:10.3233/CBM-220440

PMID:37248890

Abstract

BACKGROUND

Hepatocellular carcinoma (HCC) is one of the leading causes of cancer related deaths world over. Early diagnosis and effective treatment monitoring significantly improves patients' outcomes. FKBP11 gene is highly expressed in HCC and could play a role in its development, early diagnosis and treatment.

OBJECTIVE

This study aimed to evaluate the expression of FKBP11 in HCC, its correlation with patients' clinical characteristics and potential role in HCC development.

METHODS

Expression was determined by bioinformatics analysis, quantitative real-time PCR, western blot, and immunohistochemistry. CCK-8, Transwell and wound healing assays were used to investigate involvement in HCC development.

RESULTS

FKBP11 was significantly upregulated in HCC cells, tissues and blood (all p< 0.001). Its receiver operator characteristic (ROC) curve had an AUC of 0.864 (95% CI: 0.823-0.904), at a sensitivity of 0.86 and specificity of 0.78 indicating a good diagnostic potential in HCC. Its expression was markedly reduced after surgery (p< 0.0001), indicating a potential application in HCC treatment follow-up. Knockdown of FKBP11 in HCC cells attenuated proliferation and migration, suggesting a possible role in HCC pathogenesis.

CONCLUSION

This study thus found that FKBP11 is upregulated in HCC, and the upregulation promotes HCC development. FKBP11 levels are significantly reduced post-surgery and could be a potential diagnostic and prognostic marker for HCC.

摘要

背景

肝细胞癌（HCC）是全球导致癌症相关死亡的主要原因之一。早期诊断和有效治疗监测显著改善了患者的预后。FKBP11 基因在 HCC 中高度表达，可能在其发生、早期诊断和治疗中发挥作用。

目的

本研究旨在评估 FKBP11 在 HCC 中的表达及其与患者临床特征的相关性，并探讨其在 HCC 发生发展中的潜在作用。

方法

采用生物信息学分析、实时定量 PCR、Western blot 和免疫组织化学方法检测 FKBP11 的表达。通过 CCK-8、Transwell 和划痕愈合实验研究其在 HCC 发展中的作用。

结果

FKBP11 在 HCC 细胞、组织和血液中均显著上调（均 p<0.001）。其受试者工作特征（ROC）曲线的 AUC 为 0.864（95%CI：0.823-0.904），灵敏度为 0.86，特异性为 0.78，表明其在 HCC 诊断中有较好的应用潜力。手术后其表达明显降低（p<0.0001），表明其在 HCC 治疗随访中有潜在的应用价值。在 HCC 细胞中敲低 FKBP11 可减弱增殖和迁移，提示其在 HCC 发病机制中可能发挥作用。