哺乳动物精原干细胞的病毒转导。

Viral Transduction of Mammalian Spermatogonial Stem Cells.

机构信息

Department of Surgery, Weill Cornell Medicine, New York, NY, USA.

出版信息

Methods Mol Biol. 2023;2656:211-225. doi: 10.1007/978-1-0716-3139-3_12.

Abstract

Lentiviral vectors have been major tools for genetic manipulation of spermatogonial stem cells (SSCs) in vitro. Adeno-associated viral vectors are promising emerging tools for in vivo SSC transduction that are less invasive, compared to lentivirus, since AAV DNA is not integrated into the host genome and the host genome remains intact. In this chapter, we describe protocols using lentiviral and adeno-associated viral vectors to transduce SSCs in vitro and vivo, respectively.

摘要

慢病毒载体是体外遗传操作精原干细胞（SSC）的主要工具。与慢病毒相比，腺相关病毒载体作为一种有前途的新型体内 SSC 转导工具，其侵袭性更低，因为 AAV DNA 不会整合到宿主基因组中，并且宿主基因组保持完整。在本章中，我们分别描述了使用慢病毒和腺相关病毒载体在体外和体内转导 SSC 的方案。