基于 LC-ESI-MS/MS 的巴氏杀菌和喷雾干燥对山阴羊乳脂组学的影响。
Lipidomics of Sannen goat milk subjected to pasteurization and spray drying based on LC-ESI-MS/MS.
机构信息
Department of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
Department of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
出版信息
Food Res Int. 2023 Jul;169:112841. doi: 10.1016/j.foodres.2023.112841. Epub 2023 Apr 17.
The purpose of this study was to evaluate the effects of pasteurization and spray drying on goat milk lipids by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and multiple variable statistics. A total of 1061 lipids assigned to 29 subclasses in raw and thermal-treated groups were identified. One hundred and 85 different lipids (DLs) (VIP ≥ 1 and |LogFC| ≥ 1.0) were selected from pairwise comparisons of goat milk by different treatments. Glycerophospholipids were the most affected subclasses by thermal processes, especially by spray drying. Five potential lipid markers [(DG (16:1_18:0), TG (18:1_22:1_18:2), Cer (t17:2/31:0), LPC (0:0/20:0), and LPS (20:0/0:0] were used to distinguish different treated goat milk. Moreover, glycerophospholipid metabolism was the primary pathway of DLs. These results would provide more details of lipid profiles in thermally treated (pasteurization and spray drying) goat milk.
本研究旨在通过液相色谱-电喷雾串联质谱（LC-ESI-MS/MS）和多变量统计评估巴氏杀菌和喷雾干燥对羊奶脂质的影响。在原始组和热处理组中鉴定出 29 个亚类的 1061 种脂质。通过不同处理的羊奶两两比较，从 1085 种不同的脂质（DLs）（VIP≥1 和 |LogFC|≥1.0）中选择了 185 种不同的脂质（DLs）。甘油磷脂是受热过程影响最大的亚类，尤其是喷雾干燥。使用 5 种潜在的脂质标志物[DG（16：1_18：0），TG（18：1_22：1_18：2），Cer（t17：2/31：0），LPC（0：0/20：0）和 LPS（20：0/0：0]来区分不同处理的羊奶。此外，甘油磷脂代谢是 DLs 的主要途径。这些结果将为热处理（巴氏杀菌和喷雾干燥）羊奶中的脂质图谱提供更详细的信息。
Zotero 插件