Department of Life Science, Graduate School of Humanities and Sciences, Ochanoizu University, Bunkyo-ku, Tokyo 112-8610, Japan.
Department of Biology, Ochanoizu University, Bunkyo-ku, Tokyo 112-8610, Japan.
Zoolog Sci. 2023 Jun;40(3):235-245. doi: 10.2108/zs220099.
Frequent coral bleaching has drawn attention to the mechanisms of coral dinoflagellate endosymbiosis. Owing to the difficulty of rearing corals in the laboratory, model symbiosis systems are desired. The sea anemone , hosting clade B1 of the genus , has long been studied as a model system; however, a single species is insufficient for comparative studies and thus provides only limited resources for symbiosis research, especially regarding the specificity of host-symbiont associations. We established a clonal strain of the sea anemone , whose symbiont was identified as a novel subclade of (clade A) using a novel feeding method. We also developed a method to efficiently bleach various sea anemone species using a quinoclamine-based herbicide. Bleached polyps were vital and able to reproduce asexually, exhibiting no signs of harmful effects of the drug treatment. Pilot studies have suggested that host-symbiont specificity is influenced by multiple steps differently in and . RNAseq analyses of showed that multiple genes were expressed in the symbiotic state, which have been suggested to function in the transport of sterols from symbionts to host cells. These results reveal the usefulness of in comparative studies of cnidarian-algal symbiosis.
频繁的珊瑚白化现象引起了人们对珊瑚虫共生机制的关注。由于在实验室中培养珊瑚较为困难，因此需要建立模型共生系统。海葵长期以来一直被作为一种模型系统进行研究，其共生体属于 clade B1 的 属；然而，单一物种对于比较研究来说是不够的，因此为共生研究提供的资源也很有限，特别是在宿主-共生体关联的特异性方面。我们建立了一株海葵的克隆株，其共生体被鉴定为 clade A 的一个新的亚群（clade A），我们使用了一种新的饲养方法。我们还开发了一种使用奎诺氯胺类除草剂高效漂白各种海葵物种的方法。漂白后的海葵息肉仍然具有活力并能够进行无性繁殖，没有表现出药物处理的有害影响的迹象。初步研究表明，在 和 中，宿主-共生体的特异性受到多个步骤的不同影响。对 进行 RNAseq 分析表明，多个 基因在共生状态下表达，这些基因被认为在从共生体向宿主细胞运输固醇方面发挥作用。这些结果表明 在刺胞动物-藻类共生的比较研究中具有一定的作用。
