College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
Int J Biol Macromol. 2023 Jul 31;244:125104. doi: 10.1016/j.ijbiomac.2023.125104. Epub 2023 May 29.
In this study, we used succinic anhydride (SA) acylation and dextran (DX) glycosylation modified soybean isolate protein (SPI) to develop self-assembled SPI-SA-DX adduct-based nanogels. Degree of modification, SDS-PAGE, and FT-IR studies showed that the amino group of the SPI was replaced by hydrophilic dextran and succinic acid carboxyl groups. Dextran chain and anhydride group attachment to the soybean protein surface enhanced hydrophilicity and spatial site blocking. Modification-induced protein structure unfolding, free sulfhydryl groups to be converted to disulfide bonds, and reduced surface hydrophobicity (H). H was lowest at 33,750 ± 1008.29 when SA content = 10 % protein content (SPI-SA-DX). The nanometer gel based on SPI-SA-DX had the maximum turbidity and clear transparent solution without precipitation. Its particle size and polymer dispersibility index (PDI) were also the smallest, with values of (106.87 ± 4.51) nm and 0.21 ± 0.009, respectively. Transmission electron microscopy showed that nanogels had subspherical shell-core structures. Nanogels were stable under different pH, ionic strength, high temperature, and storage conditions.
在这项研究中，我们使用琥珀酸酐（SA）酰化和葡聚糖（DX）糖基化修饰大豆分离蛋白（SPI），开发了自组装的 SPI-SA-DX 加合物基纳米凝胶。修饰程度、SDS-PAGE 和 FT-IR 研究表明，SPI 的氨基被亲水性葡聚糖和琥珀酸羧基取代。葡聚糖链和酸酐基团附着在大豆蛋白表面，增强了亲水性和空间位阻。修饰诱导的蛋白质结构展开，游离巯基转化为二硫键，表面疏水性（H）降低。当 SA 含量为 10%蛋白质含量（SPI-SA-DX）时，H 最低，为 33750±1008.29。基于 SPI-SA-DX 的纳米凝胶具有最大浊度和清澈透明的溶液，没有沉淀。其粒径和聚合物分散指数（PDI）也最小，分别为（106.87±4.51）nm 和 0.21±0.009。透射电子显微镜显示纳米凝胶具有亚球形核壳结构。纳米凝胶在不同的 pH、离子强度、高温和储存条件下均稳定。
Zotero 插件