Children's Medical Center Research Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Methods Mol Biol. 2023;2675:133-148. doi: 10.1007/978-1-0716-3247-5_11.
Oxidation of glutathione (GSH) to its disulfide dimer (GSSG) is the major mechanism by which cells balance reactive oxygen species (ROS) and mitigate oxidative stress. Thus, measuring the ratio of GSH/GSSG is an ideal way to assess oxidative stress within a cell. Quantitative mass spectrometry offers an ideal method to measure the GSH/GSSG ratio and can be applied to a variety of biological matrices and disease models. The following chapter details the design, optimization, and execution of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay to measure the GSH/GSSG ratio.
谷胱甘肽（GSH）氧化为其二硫键二聚体（GSSG）是细胞平衡活性氧（ROS）和减轻氧化应激的主要机制。因此，测量 GSH/GSSG 的比值是评估细胞内氧化应激的理想方法。定量质谱提供了测量 GSH/GSSG 比值的理想方法，可应用于多种生物基质和疾病模型。本章详细介绍了设计、优化和执行液相色谱-串联质谱（LC-MS/MS）测定 GSH/GSSG 比值的方法。
