Luu Vinh Quang, Nguyen Thuong Huyen, Le Minh Duc, Grismer Jesse L, Ha Hong Bich, Sitthivong Saly, Hoang Tuoi Thi, Grismer L Lee
Faculty of Forest Resources and Environmental Management, Vietnam National University of Forestry, Xuan Mai, Chuong My, Hanoi, Vietnam.
Herpetology Laboratory, Department of Biology, La Sierra University, 4500 Riverwalk Parkway, Riverside, California 92505, USA.
Zookeys. 2023 May 23;1163:143-176. doi: 10.3897/zookeys.1163.101230. eCollection 2023.
Integrated analyses using maximum likelihood (ML), Bayesian inference (BI), principal component analysis (PCA), discriminate analysis of principal components (DAPC), multiple factor analysis (MFA), and analysis of variance (ANOVA) recovered two new diagnosable species of gekkonid lizards in the genus , one from the Central Highlands, Gia Lai Province, Vietnam and another from the Vientiane Province, Laos. Phylogenetic analyses based on the mitochondrial NADH dehydrogenase subunit 2 gene (ND2) and adjacent tRNAs showed that is the sister species of from Dong Nai Province, Vietnam and is nested within a clade that also includes the sister species and . is the sister species to from Khammouane Province, Laos and is embedded in a clade with , , and undescribed species from Thailand. Multivariate (PCA, DAPC, and MFA) and univariate (ANOVA) analyses using combinations of 15 meristic (scale counts), six morphometric (measurements), and five categorical (color pattern and morphology) characters from 44 specimens encompassing all eight species of from Vietnam and Laos clearly illustrate and are statistically different and discretely diagnosable from all closely related species of . These integrative analyses also highlight additional taxonomic issues that remain unresolved within and the need for additional studies. The discovery of these new species further emphasizes the underappreciated herpetological diversity of the genus and illustrates the continued need for field work in these regions.
使用最大似然法(ML)、贝叶斯推断(BI)、主成分分析(PCA)、主成分判别分析(DAPC)、多因素分析(MFA)和方差分析(ANOVA)进行的综合分析,在该属中发现了两种新的可诊断的壁虎科蜥蜴物种,一种来自越南嘉莱省的中部高地,另一种来自老挝万象省。基于线粒体烟酰胺腺嘌呤二核苷酸脱氢酶亚基2基因(ND2)和相邻tRNA的系统发育分析表明,[物种名1]是越南同奈省[物种名2]的姐妹物种,并且嵌套在一个进化枝中,该进化枝还包括姐妹物种[物种名3]和[物种名4]。[物种名5]是老挝甘蒙省[物种名6]的姐妹物种,并与[物种名7]、[物种名8]以及来自泰国的未描述物种一起嵌入一个进化枝中。使用来自越南和老挝的该属所有8个物种的44个标本的15个数量性状(鳞片计数)、6个形态测量性状(测量值)和5个分类性状(颜色模式和形态)组合进行的多变量(PCA、DAPC和MFA)和单变量(ANOVA)分析清楚地表明,[物种名1]和[物种名5]在统计学上与该属所有密切相关的物种不同,并且可以明确诊断。这些综合分析还突出了该属内仍未解决的其他分类学问题以及进行更多研究的必要性。这些新物种的发现进一步强调了该属未得到充分认识的爬行动物多样性,并说明了在这些地区持续开展野外工作的必要性。