1 年每日蛋白质补充和体育锻炼对健康丹麦老年人肌肉蛋白质合成率和肌肉代谢组的影响：一项随机对照试验。

Effect of 1-year daily protein supplementation and physical exercise on muscle protein synthesis rate and muscle metabolome in healthy older Danes: a randomized controlled trial.

机构信息

Department of Orthopedic Surgery M, Institute of Sports Medicine Copenhagen M81, Bispebjerg Hospital, Building 8, Level 1, Nielsine Nielsens Vej 11, 2400, Copenhagen NV, Denmark.

Department of Food Sciences, University of Copenhagen, Copenhagen, Denmark.

出版信息

Eur J Nutr. 2023 Sep;62(6):2673-2685. doi: 10.1007/s00394-023-03182-0. Epub 2023 Jun 2.

DOI:10.1007/s00394-023-03182-0

PMID:37266586

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10421766/

Abstract

BACKGROUND

The skeletal muscle mass decreases with age and the responsiveness of aging muscles' protein synthesis rate (MPS) to protein intake seems to deteriorate.

OBJECTIVE

This study investigated the impact of 12 months of protein supplementation with or without physical exercise training on the basal and postprandial MPS and the skeletal muscle metabolome of healthy older Danes (> 65 years, 29 females/37 males).

METHODS

Subjects were randomized to follow one of five intervention groups: (1) carbohydrate, (2) collagen protein, (3) whey protein, (4) home-based light resistance training with whey protein, and (5) center-based heavy-load resistance training with whey protein. Before and after the intervention, a tracer infusion trial was conducted to measure basal and postprandial MPS in response to intake of a cocktail consisting of 20 g whey hydrolysate + 10 g glucose. In addition, the skeletal muscle metabolome was measured using gas chromatography-mass spectrometry (GC-MS) at basal state and 4 h after the intake of the cocktail.

RESULTS

One year of daily protein or carbohydrate supplementation did not alter the basal and protein-stimulated postprandial muscle protein synthesis rate or the muscle metabolome of healthy older Danes. Basal MPS (%/h) at baseline for all subjects were 0.0034 ± 0,011 (mean ± SD). In contrast to previous studies, no difference was observed in basal MPS between males and females (p = 0.75). With the developed untargeted GC-MS methodology, it was possible to detect and tentatively annotate > 70 metabolites from the human skeletal muscle samples.

CONCLUSION

One year of protein supplementation in comparison to an isocaloric-control supplement seems to affect neither the MPS at basal or postprandial state nor the skeletal muscle metabolome.

CLINICAL TRIAL REGISTRY

Number: NCT02115698, clinicaltrials.gov/ct2/show/NCT02115698.

摘要

背景

骨骼肌质量随年龄增长而减少，衰老肌肉的蛋白质合成率（MPS）对蛋白质摄入的反应似乎恶化。

目的

本研究调查了 12 个月的蛋白质补充（含或不含体育锻炼训练）对健康丹麦老年人（>65 岁，29 名女性/37 名男性）基础和餐后 MPS 以及骨骼肌代谢组的影响。

方法

受试者随机分为以下五个干预组之一：（1）碳水化合物，（2）胶原蛋白，（3）乳清蛋白，（4）基于家庭的轻阻力训练+乳清蛋白，和（5）基于中心的重负荷阻力训练+乳清蛋白。在干预前后，进行示踪剂输注试验，以测量摄入 20g 乳清水解物+10g 葡萄糖混合物后基础和餐后 MPS。此外，使用气相色谱-质谱法（GC-MS）在基础状态和摄入混合物后 4 小时测量骨骼肌代谢组。

结果

一年的日常蛋白质或碳水化合物补充并未改变健康丹麦老年人的基础和蛋白质刺激的餐后肌肉蛋白质合成率或肌肉代谢组。所有受试者的基础 MPS（%/h）在基线时为 0.0034±0.011（平均值±标准差）。与之前的研究不同，男性和女性之间的基础 MPS 没有差异（p=0.75）。使用开发的非靶向 GC-MS 方法，有可能从人体骨骼肌样本中检测并暂定注释>70 种代谢物。