原发性胆汁性胆管炎和类风湿关节炎患者的血清学标志物：一项突尼斯研究。
Serological markers of rheumatoid arthritis in patients with primary biliary cholangitis and the vice versa: A Tunisian study.
机构信息
Laboratory of Immunology, Farhat Hached University Hospital, Sousse, Tunisia; Faculty of Pharmacy, Department of Immunology, University of Monastir, Monastir, Tunisia; Research Laboratory for "Epidemiology and Immunogenetics of Viral Infections" (LR14SP02), Sahloul University Hospital, University of Sousse, Sousse, Tunisia.
High School of Sciences and Techniques of Health, Tunis El Manar University, Tunis, Tunisia; Research Unit "Obesity: Etiopathology and Treatment, UR18ES01", National Institute of Nutrition and Food Technology, Tunis, Tunisia.
出版信息
Immunobiology. 2023 Jul;228(4):152398. doi: 10.1016/j.imbio.2023.152398. Epub 2023 May 29.
BACKGROUND
Primary biliary cholangitis (PBC) is an autoimmune disease of the liver characterized by destructive lymphocytic cholangitis and anti-mitochondrial antibodies (AMA). Anti-gp210 and anti-Sp100, are used for the diagnosis of PBC in AMA-negative PBC patients. Patients with PBC have a propensity to have an extrahepatic manifestation which is especially autoimmune.
OBJECTIVE
We aimed to determine the frequency of serological markers of rheumatoid arthritis (RA) (CCP-Ab or RF) in PBC patients and to do the vice versa.
METHODS
Our PBC study included 70 patients with PBC and 80 healthy blood donors (HBD) and our RA study included 75 patients with RA and 75 HBD. Anti-cyclic citrullinated peptide antibodies (CCP-Ab) and rheumatoid factor (RF) were performed by indirect ELISA. AMA, anti-Sp100 and anti-gp210 were determined by indirect immunofluorescence.
RESULTS
RA autoantibodies (CCP-Ab or RF) were more frequent in PBC patients than in HBD (65.7% vs. 8.7% p 〈10). CCP-Ab were significantly more frequent in patients than in controls (15.7% vs. 2.5%; p = 0.004). Nine patients had both CCP-Ab and RF vs. none of controls (12.8% vs. 0%; p = 0.001). RF were detected in 45 patients with PBC and in 5 HBD (64.3% vs. 6.2%; p 〈10). In PBC patients, RF were more frequent than CCP-Ab (64.3% vs. 15.7%; p 〈10). RF-IgG were present in 18.5% of patients; RF-immunoglobulin (Ig) A in 34.3% and RF-IgM in 54.3%. These frequencies were significantly higher than those found in control group (1.2% for RF-IgG (p 〈10); 0% for RF-IgA (p 〈10); and 6.2% for RF-IgM (p 〈10)). In our PBC patients, RF-IgA were more frequent than RF-IgG (34.3% vs. 18.5%; p = 0.03) and than CCP-Ab (34.3% vs. 15.7%; p = 0.01). Six patients had only RF-IgA versus none of the control group (8.6% vs. 0%; p = 0.01). AMA, anti-Sp100 and anti-gp 210 were absent in all RA patients.
CONCLUSIONS
Serological markers of RA were more frequent in PBC patients than in HBD and the vice versa was not true.
背景
原发性胆汁性胆管炎（PBC）是一种肝脏自身免疫性疾病，其特征为破坏性淋巴细胞性胆管炎和抗线粒体抗体（AMA）。抗 gp210 和抗 Sp100 用于诊断 AMA 阴性 PBC 患者中的 PBC。PBC 患者有发生肝外表现的倾向，尤其是自身免疫性的。
目的
我们旨在确定 PBC 患者血清类风湿关节炎（RA）标志物（抗环瓜氨酸肽抗体 [CCP-Ab] 或 RF）的频率，并反之亦然。
方法
我们的 PBC 研究包括 70 例 PBC 患者和 80 名健康献血者（HBD），我们的 RA 研究包括 75 例 RA 患者和 75 名 HBD。通过间接 ELISA 检测抗环瓜氨酸肽抗体（CCP-Ab）和类风湿因子（RF）。通过间接免疫荧光法测定 AMA、抗 Sp100 和抗 gp210。
结果
与 HBD 相比，PBC 患者中 RA 自身抗体（CCP-Ab 或 RF）更为常见（65.7%比 8.7%，p〈10）。与对照组相比，患者中 CCP-Ab 明显更为常见（15.7%比 2.5%；p=0.004）。9 例患者同时存在 CCP-Ab 和 RF，而对照组中无一例存在（12.8%比 0%；p=0.001）。45 例 PBC 患者和 5 例 HBD 检测到 RF（64.3%比 6.2%；p〈10）。在 PBC 患者中，RF 比 CCP-Ab 更为常见（64.3%比 15.7%；p〈10）。18.5%的患者存在 RF-IgG；34.3%存在 RF-免疫球蛋白（Ig）A，54.3%存在 RF-IgM。这些频率明显高于对照组（RF-IgG 为 1.2%（p〈10）；RF-IgA 为 0%（p〈10）；RF-IgM 为 6.2%（p〈10））。在我们的 PBC 患者中，RF-IgA 比 RF-IgG 更为常见（34.3%比 18.5%；p=0.03），也比 CCP-Ab 更为常见（34.3%比 15.7%；p=0.01）。6 例患者仅存在 RF-IgA，而对照组中无一例存在（8.6%比 0%；p=0.01）。所有 RA 患者均不存在 AMA、抗 Sp100 和抗 gp 210。
结论
与 HBD 相比，PBC 患者中 RA 血清标志物更为常见，反之则不然。
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