Liu Xibo, Chen Lirong, Wen Fei, Zheng Shu, Ge Weiting
Department of Pathology, Shaoxing People's Hospital, No. 568, Zhongxing North Road, Shaoxing, 312000, Zhejiang Province, China.
Department of Pathology, The Second Affiliated Hospital of Zhejiang University School of Medicine, No.88 Jiefang Road, Hangzhou, 310009, China.
Clin Exp Med. 2023 Nov;23(7):3995-4001. doi: 10.1007/s10238-023-01096-z. Epub 2023 Jun 6.
Purpose Forkhead box (FOX) family proteins regulate transcription and DNA repair and are involved in cell growth, differentiation, embryogenesis, and lifespan. The transcription factor FOXE1 is a member of the FOX family. The relationship between the expression level of FOXE1 and colorectal cancer (CRC) prognosis remains controversial. It is vital to verify the relationship between FOXE1 expression and the prognosis of patients with CRC. Methods We constructed a tissue microarray containing 879 primary colorectal cancer tissues and 203 normal mucosa samples. The tumor and normal mucosa tissues were stained with FOXE1 by immunohistochemistry, and the staining results were divided into two groups: high expression group and low expression group. Chi-square test was performed for the classification variable of the difference between FOXE1 expression levels and clinicopathological parameters. The survival curve was calculated according to the Kaplan-Meier method and the logarithmic rank test. The Cox proportional risk regression model was used for multivariate analysis of prognostic factors in patients with CRC.Results The expression level of FOXE1 in colorectal cancer was higher than that in the normal mucosa adjacent to cancer, although the difference was not significant. However, the expression of FOXE1 was correlated with tumor size, T stage, N stage, M stage, and pTNM stage. Univariate and multivariate analyses suggested that FOXE1 could be used as an independent prognostic factor in patients with CRC. Conclusions FOXE1 may be a potential independent prognostic factor for colorectal cancer patients.
目的 叉头框（FOX）家族蛋白调节转录和DNA修复，并参与细胞生长、分化、胚胎发生和寿命调节。转录因子FOXE1是FOX家族的成员之一。FOXE1表达水平与结直肠癌（CRC）预后之间的关系仍存在争议。验证FOXE1表达与CRC患者预后之间的关系至关重要。方法 我们构建了一个包含879个原发性结直肠癌组织和203个正常黏膜样本的组织芯片。通过免疫组织化学法用FOXE1对肿瘤组织和正常黏膜组织进行染色，并将染色结果分为两组：高表达组和低表达组。对FOXE1表达水平与临床病理参数之间差异的分类变量进行卡方检验。根据Kaplan-Meier法和对数秩检验计算生存曲线。采用Cox比例风险回归模型对CRC患者的预后因素进行多因素分析。结果 结直肠癌中FOXE1的表达水平高于癌旁正常黏膜，尽管差异不显著。然而，FOXE1的表达与肿瘤大小、T分期、N分期、M分期和pTNM分期相关。单因素和多因素分析表明，FOXE1可作为CRC患者的独立预后因素。结论 FOXE1可能是结直肠癌患者潜在的独立预后因素。
