Department of Biotechnology, Inland Norway University of Applied Sciences, Hamar, Norway.
Norsvin SA, Hamar, Norway.
Zygote. 2023 Oct;31(5):441-450. doi: 10.1017/S0967199423000308. Epub 2023 Jun 8.
Commercial application of embryo transfer in pig breeding is dependent on the storage of embryos. The aim of this study was to assess the embryo quality of -produced blastocysts after 3 h liquid storage at 37°C in CO-free medium by evaluating morphology, developmental capacity and apoptosis. Blastocysts at days 5 and 6 post-fertilization were randomly allocated to the storage group (HEPES-buffered NCSU-23 medium including bovine serum albumin in a portable embryo transport incubator at 37°C) or a control group (porcine blastocyst medium in a conventional culture incubator). Thereafter, blastocysts were evaluated for morphology and stained to assess apoptosis straight after the 3 h storage period or after a further 24 h conventional incubation. There was no significant difference between the storage and control group after 3 h storage and the further 24 h conventional incubation for any of the parameters, nor for apoptosis straight after the 3 h storage. Embryos that reached the blastocyst stage at day 5 showed less apoptosis (6.6% vs 10.9%, = 0.01) and a trend for a higher rate of developmental capacity (70.6% vs 51.5%, = 0.089) than embryos reaching the blastocyst stage on day 6. In conclusion, -produced porcine blastocysts can be stored for 3 h at physiological temperature in transportable incubators using a CO-independent medium without compromising quality.
商业应用胚胎移植在猪养殖取决于胚胎的储存。本研究的目的是评估生产的胚胎在 37°C 无 CO 培养基中 3 小时液体储存后的胚胎质量，通过评估形态、发育能力和凋亡。在受精后 5 天和 6 天的囊胚被随机分配到储存组（含有牛血清白蛋白的 HEPES 缓冲 NCSU-23 培养基，在便携式胚胎运输培养箱中在 37°C 下）或对照组（在常规培养箱中的猪囊胚培养基）。此后，在 3 小时储存期后或进一步常规孵育 24 小时后，对囊胚进行形态评估和染色以评估凋亡。在 3 小时储存和进一步 24 小时常规孵育后，任何参数之间均无显著差异，3 小时储存后立即进行凋亡也无差异。在第 5 天达到囊胚阶段的胚胎显示出较低的凋亡率（6.6%对 10.9%， = 0.01）和较高的发育能力趋势（70.6%对 51.5%， = 0.089）比第 6 天达到囊胚阶段的胚胎。总之，生产的猪囊胚可以在生理温度下在可运输的培养箱中使用 CO 独立的培养基储存 3 小时而不会降低质量。
