School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China; Key Lab of Northwest Water Resource, Environment and Ecology, Ministry of Education, Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China; Key Lab of Northwest Water Resource, Environment and Ecology, Ministry of Education, Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China; International Science and Technology Cooperation Center for Urban Alternative Water Resources Development, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
Chemosphere. 2023 Sep;335:139151. doi: 10.1016/j.chemosphere.2023.139151. Epub 2023 Jun 6.
Microplastics (MPs) could serve as substrates for microbial colonization and biofilm formation. However, research on the effects of different types of microplastics and natural substrates on biofilm formation and community structure in the presence of antibiotic-resistant bacteria (ARB) is limited. In this study, we employed by means of microcosm experiments to analyze the situation of biofilms conditions, bacterial resistance patterns, antibiotic resistance genes (ARGs) distribution, and bacterial community on different substrates using microbial cultivation, high throughtput sequencing and PCR. The result showed that biofilms on different substrates markedly increased with time, with MPs surfaces formed more biofilm than stone. Analyses of antibiotic resistant showed negligible differences in the resistance rate to the same antibiotic at 30 d, but tetB would be selectively enriched on PP and PET. The microbial communities associated with biofilms on MPs and stones exhibited variations during different stages of formation. Notably, phylum WPS-2 and Epsilonbacteraeota were identified as the dominant microbiomes of biofilms on MPs and stones at 30 d, respectively. Correlation analysis suggested that WPS-2 could potentially be a tetracycline-resistant bacterium, while Epsilonbacteraeota did not correlate with any detected ARB. Our results emphasized the potential threat posed by MPs as attachment carriers for bacteria, particularly ARB, in aquatic environments.
微塑料（MPs）可以作为微生物定殖和生物膜形成的基质。然而，关于不同类型的微塑料和天然基质对存在抗生素耐药菌（ARB）的生物膜形成和群落结构的影响的研究有限。在这项研究中，我们通过微宇宙实验，利用微生物培养、高通量测序和 PCR 等手段，分析了不同基质上生物膜条件、细菌耐药模式、抗生素耐药基因（ARGs）分布和细菌群落的情况。结果表明，不同基质上的生物膜随时间显著增加， MPs 表面形成的生物膜比石头多。抗生素耐药性分析表明，在 30d 时，对同一抗生素的耐药率差异可以忽略不计，但 tetB 会在 PP 和 PET 上选择性富集。与 MPs 和石头上生物膜相关的微生物群落在形成的不同阶段表现出变化。值得注意的是，在 30d 时，厚壁菌门 WPS-2 和 Epsilonbacteraeota 分别被鉴定为 MPs 和石头上生物膜的主要微生物组。相关性分析表明，WPS-2 可能是一种四环素耐药菌，而 Epsilonbacteraeota 与任何检测到的 ARB 都没有相关性。我们的结果强调了 MPs 作为水生环境中细菌，特别是 ARB 的附着载体所带来的潜在威胁。
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