Chaplin H, Malecek A C, Miller R E, Bell C E, Gray L S, Hunter V L
Am J Vet Res. 1986 Jun;47(6):1313-20.
To investigate the syndrome of acute intravascular hemolytic anemia in the black rhinoceros (Diceros bicornis), laboratory techniques used in the differential diagnosis of hemolytic anemias were performed on blood samples from 6 black rhinoceroses: 3 nonrelated healthy rhinoceroses, 1 rhinoceros with iron deficiency anemia, and 2 rhinoceroses with intravascular hemolysis. Osmotic fragility, erythrocyte membrane protein composition, hemoglobin electrophoresis, and hemoglobin stability did not distinguish between healthy and affected (anemia or hemolysis) rhinoceroses. Polyclonal antiglobulin reagents were prepared in rabbits, using whole rhinoceros serum and purified rhinoceros immunoglobulin G. These reagents were nonreactive against erythrocytes of the healthy and iron-deficient rhinoceroses. Reactions with RBC from the rhinoceros with fatal hemolytic anemia indicated increased membrane coating by the third component of complement; this was not evident in a second rhinoceros that survived a hemolytic event.
为了研究黑犀牛（双角犀属）的急性血管内溶血性贫血综合征，对6头黑犀牛的血样进行了用于溶血性贫血鉴别诊断的实验室技术检测：3头无亲缘关系的健康犀牛、1头缺铁性贫血的犀牛和2头血管内溶血的犀牛。渗透脆性、红细胞膜蛋白组成、血红蛋白电泳和血红蛋白稳定性在健康犀牛与患病（贫血或溶血）犀牛之间并无差异。使用全犀牛血清和纯化的犀牛免疫球蛋白G在兔体内制备了多克隆抗球蛋白试剂。这些试剂与健康和缺铁犀牛的红细胞无反应。与致命性溶血性贫血犀牛的红细胞反应表明补体第三成分对膜的包被增加；而在经历溶血事件后存活的另一头犀牛中未观察到这种现象。
