Çöl Başak Gökçe, Akhan Meryem, Sancar Burcu Çakmak, Türkol Melikenur, Yıkmış Seydi, Hecer Canan
Department of Nutrition and Dietetics, İstanbul Gelisim University, Istanbul 34000, Turkey.
Department of Nutrition and Dietetics, İstanbul Esenyurt University, Istanbul 34510, Turkey.
Foods. 2023 May 27;12(11):2167. doi: 10.3390/foods12112167.
Thermosonication is a process that can be used as an alternative to thermal pasteurization by combining mild temperature and ultrasound treatments. This study evaluated the effects of verjuice on the thermosonication process and its bioactive values modeled with the RSM (response surface method). The bioactive components of verjuice were found to increase with high predictive values. Additionally, the presence and amounts of 20 free amino acids in C-VJ (untreated verjuice), P-VJ (thermally pasteurized verjuice) and TS-VJ (thermosonicated verjuice) samples were investigated. Significant ( < 0.05) differences were detected among C-VJ, P-VJ and TS-VJ samples in all free amino acid values except methionine. Although 17 free amino acids were detected at various concentrations, glycine, taurine and cystine were not found in any samples. Thirteen phenolic filters in C-VJ, P-VJ and TS-VJ samples were also examined in this study. Eight phenolic donors with various abilities were detected in the C-VJ sample, along with nine phenolic acceptors in the P-VJ sample and eleven phenolic contents in the TS-VJ sample. The content of phenolic products in the TS-VJ sample increased by 37.5% compared to the C-VJ techniques and by 22.22% compared to the P-VJ techniques. Thermosonication did not significantly affect color and physiochemical values. Panelists generally appreciated the effects of thermosonication. It is concluded that the thermosonication process is a good alternative to thermal pasteurization. The results of this study provide essential data for future in vivo studies and show that the bioactive values of verjuice can be increased by using the thermosonication process.
热超声处理是一种可通过温和温度与超声处理相结合来替代热巴氏杀菌的工艺。本研究评估了酸葡萄汁对热超声处理工艺及其用响应面法(RSM)建模的生物活性值的影响。发现酸葡萄汁的生物活性成分增加且预测值较高。此外,还研究了未处理酸葡萄汁(C-VJ)、热巴氏杀菌酸葡萄汁(P-VJ)和热超声处理酸葡萄汁(TS-VJ)样品中20种游离氨基酸的存在情况和含量。除蛋氨酸外,C-VJ、P-VJ和TS-VJ样品在所有游离氨基酸值上均检测到显著(<0.05)差异。尽管检测到17种游离氨基酸浓度各异,但所有样品中均未发现甘氨酸、牛磺酸和胱氨酸。本研究还检测了C-VJ、P-VJ和TS-VJ样品中的13种酚类成分。在C-VJ样品中检测到8种具有不同活性的酚类供体,P-VJ样品中有9种酚类受体,TS-VJ样品中有11种酚类成分。与C-VJ工艺相比,TS-VJ样品中酚类产物的含量增加了37.5%,与P-VJ工艺相比增加了22.22%。热超声处理对颜色和理化值没有显著影响。评审员普遍认可热超声处理的效果。得出的结论是,热超声处理工艺是热巴氏杀菌的良好替代方法。本研究结果为未来的体内研究提供了重要数据,并表明使用热超声处理工艺可提高酸葡萄汁的生物活性值。