建立一种 LC-MS/MS 方法用于检测人尿液中的多种真菌毒素。
Development of an LC-MS/MS method for the determination of multiple mycotoxins in human urine.
机构信息
School of Pharmacy, China Pharmaceutical University, Nanjing, P. R. China.
Academy of Forensic Science, Shanghai Key Laboratory of Forensic Medicine, Shanghai, P. R. China.
出版信息
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2023 Jul;40(7):917-927. doi: 10.1080/19440049.2023.2222842. Epub 2023 Jun 14.
A sensitive liquid chromatography-tandem mass spectrometry method was developed for the determination of 17 mycotoxins in human urine. The method incorporates a two-step liquid-liquid extraction with ethyl acetate:acetonitrile (7:1), which had good extraction recovery. The LOQs of all mycotoxins ranged from 0.1 to 1 ng/mL. Intra-day accuracy ranged from 94 to 106%, and intra-day precision ranged from 1 to 12% for all mycotoxins. Inter-day accuracy and precision were 95-105% and 2-8%, respectively. The method was successfully applied to investigate the urine levels of 17 mycotoxins from 42 volunteers. Deoxynivalenol (DON, 0.97-9.88 ng/mL) was detected in 10 (24%) urine samples and zearalenone (ZEN, 0.13-1.11 ng/mL) in 2 (5%) urine samples.
建立了一种灵敏的液相色谱-串联质谱法，用于测定人尿液中的 17 种真菌毒素。该方法采用乙酸乙酯-乙腈（7:1）两步液液萃取，具有良好的萃取回收率。所有真菌毒素的 LOQs 范围为 0.1 至 1ng/mL。所有真菌毒素的日内准确度范围为 94%至 106%，日内精密度范围为 1%至 12%。日间准确度和精密度分别为 95-105%和 2-8%。该方法成功应用于检测 42 名志愿者尿液中 17 种真菌毒素的水平。在 10 份（24%）尿液样本中检测到脱氧雪腐镰刀菌烯醇（DON，0.97-9.88ng/mL），在 2 份（5%）尿液样本中检测到玉米赤霉烯酮（ZEN，0.13-1.11ng/mL）。
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