喀布尔社区鼻腔金黄色葡萄球菌的分子特征及抗菌药物耐药性

Molecular characterization and antimicrobial resistance of nasal Staphylococcus aureus in the community of Kabul.

作者信息

Naimi Haji Mohammad, Tristan Anne, Bes Michèle, Vandenesch François, Nazari Qand Agha, Laurent Frédéric, Dupieux Céline

机构信息

Department of Microbiology, Faculty of Pharmacy, Kabul University, Kabul, Afghanistan; CIRI - Centre International de Recherche en Infectiologie, Univ Lyon, Inserm U1111, Université Claude Bernard Lyon 1, CNRS UMR5308, ENS de Lyon, Lyon, France.

CIRI - Centre International de Recherche en Infectiologie, Univ Lyon, Inserm U1111, Université Claude Bernard Lyon 1, CNRS UMR5308, ENS de Lyon, Lyon, France; Centre National de Référence des Staphylocoques, Institut des Agents Infectieux, Hospices Civils de Lyon, Lyon, France.

出版信息

J Glob Antimicrob Resist. 2023 Sep;34:18-22. doi: 10.1016/j.jgar.2023.06.001. Epub 2023 Jun 14.

DOI:10.1016/j.jgar.2023.06.001

PMID:37321392

Abstract

OBJECTIVES

This study aimed to investigate the prevalence and molecular characteristics of community methicillin-resistant Staphylococcus aureus (MRSA) nasal carriage among students at Kabul University.

METHODS

Nasal swabs were collected from anterior nares of 150 healthy non-medical students at Kabul University. Antimicrobial susceptibility testing was performed on all S. aureus isolates, and all detected MRSA isolates were then confirmed by mecA/mecC polymerase chain reaction and characterized using DNA microarray.

RESULTS

A total of 50 S. aureus strains were isolated from the anterior nares of the 150 participants. The prevalence of S. aureus and MRSA nasal carriage among Kabul students was 33.3% and 12.7%, respectively. Seven (36.8%) MRSA isolates and 8 (25.8%) methicillin-susceptible S. aureus (MSSA) isolates were multidrug-resistant (i.e. resistant to at least three different antimicrobials tested). All MRSA isolates (n = 19) were susceptible to linezolid, rifampicin, and fusidic acid. Seven MRSA clones, belonging to four clonal complexes (CCs), were identified. The most commonly identified clone was CC22-MRSA-IV TSST-1-positive, which accounted for 63.2% (12/19) of MRSA isolates. SCCmec typing showed that most MRSA strains harboured SCCmec type IV (94.7%). Thirteen (68.4%) MRSA isolates carried the TSST-1 and 5 (26.3%) PVL genes.

CONCLUSION

Our findings revealed the relatively high prevalence of MRSA nasal carriers in the community in Kabul, with the predominance of the CC22-MRSA-IV TSST-1-positive clone and frequent multidrug resistance among these isolates.

摘要

目的

本研究旨在调查喀布尔大学学生群体中甲氧西林耐药金黄色葡萄球菌（MRSA）鼻腔携带情况的流行率及分子特征。

方法

从喀布尔大学150名健康非医学专业学生的前鼻孔采集鼻拭子。对所有金黄色葡萄球菌分离株进行药敏试验，所有检测到的MRSA分离株随后通过mecA/mecC聚合酶链反应进行确认，并使用DNA微阵列进行特征分析。

结果

从150名参与者的前鼻孔中共分离出50株金黄色葡萄球菌菌株。喀布尔学生中金黄色葡萄球菌和MRSA鼻腔携带率分别为33.3%和12.7%。7株（36.8%）MRSA分离株和8株（25.8%）甲氧西林敏感金黄色葡萄球菌（MSSA）分离株对多种药物耐药（即对至少三种测试的不同抗菌药物耐药）。所有MRSA分离株（n = 19）对利奈唑胺、利福平和夫西地酸敏感。鉴定出7个属于4个克隆复合体（CCs）的MRSA克隆。最常见的克隆是CC22-MRSA-IV TSST-1阳性，占MRSA分离株的63.2%（12/19）。SCCmec分型显示，大多数MRSA菌株携带IV型SCCmec（94.7%）。13株（68.4%）MRSA分离株携带TSST-1基因，5株（26.3%）携带PVL基因。