Luine V N, Renner K J, McEwen B S
Endocrinology. 1986 Aug;119(2):874-8. doi: 10.1210/endo-119-2-874.
We investigated whether estrogenic actions of testosterone during development which mediate the suppression of feminine reproductive behavior and cyclic gonadotropin secretion also contribute to reported sex differences in the induction of choline acetyltransferase (ChAT) after estrogen priming in the diagonal band region of the preoptic area. Newborn female rats received estradiol (E2 females); newborn males received 1,4,6-androstatrien-3,17-dione (ATD), an inhibitor of aromatase (ATD males); and some of both sexes received vehicle treatment (control). In adulthood, feminine sexual behavior (lordosis) was tested after E2 plus progesterone priming. The neonatal treatments reversed the sex-specific response pattern; E2 females were defeminized and displayed minimal lordosis, as did control males, while ATD males showed maximal lordosis, as did control females. E2 was then administered, and ChAT activity was measured in the horizontal and vertical nuclei of the diagonal bands (hDB and vDB, respectively). Controls exhibited the normal sex-specific response to E2. Females showed increased ChAT activity in the hDB and unaltered activity in the vDB: males had unaltered ChAT activity in the hDB and decreased activity in the vDB. In neonatally treated males and females, ChAT activity after E2 administration was not altered from the normal sex-specific pattern in the hDB, i.e. all females showed increased hDB ChAT after E2, and no male responded. In the vDB, groups defeminized in terms of lordosis (E2 females and control males) showed higher ChAT activity in the absence of E2 priming, and E2 treatment decreased vDB ChAT in these groups. In addition, ATD males showed a unique response to E2 in the vDB, namely increased ChAT activity. Although neonatal E2 and ATD treatments did not completely reverse the sex-specific pattern of E2 priming on ChAT activity, the results obtained suggest that a net increase in diagonal band cholinergic function, as indexed by increased ChAT activity after E2 priming, may contribute to the ability of hormones to induce lordosis and/or LH surges.
我们研究了睾酮在发育过程中的雌激素作用，这种作用介导了对雌性生殖行为和周期性促性腺激素分泌的抑制，是否也导致了在视前区斜角带区域经雌激素预处理后，胆碱乙酰转移酶（ChAT）诱导方面所报道的性别差异。新生雌性大鼠接受雌二醇（E2雌性）；新生雄性大鼠接受芳香化酶抑制剂1,4,6 - 雄甾三烯 - 3,17 - 二酮（ATD，ATD雄性）；部分雌雄大鼠接受溶剂处理（对照）。成年后，在E2加孕酮预处理后测试雌性性行为（脊柱前凸）。新生期处理逆转了性别特异性反应模式；E2雌性大鼠表现出雌性化缺失，脊柱前凸极少，对照雄性大鼠也是如此，而ATD雄性大鼠表现出最大程度的脊柱前凸，对照雌性大鼠也是如此。然后给予E2，并分别在斜角带的水平核和垂直核（分别为hDB和vDB）中测量ChAT活性。对照组对E2表现出正常的性别特异性反应。雌性大鼠hDB中的ChAT活性增加，vDB中的活性未改变；雄性大鼠hDB中的ChAT活性未改变，vDB中的活性降低。在新生期接受处理的雄性和雌性大鼠中，给予E2后hDB中的ChAT活性与正常性别特异性模式相比未改变，即所有雌性大鼠在给予E2后hDB中的ChAT活性增加，而没有雄性大鼠有反应。在vDB中，在脊柱前凸方面表现出雌性化缺失的组（E2雌性大鼠和对照雄性大鼠）在未进行E2预处理时ChAT活性较高，E2处理使这些组的vDB中ChAT活性降低。此外，ATD雄性大鼠在vDB中对E2表现出独特反应，即ChAT活性增加。尽管新生期E2和ATD处理并未完全逆转E2预处理对ChAT活性的性别特异性模式，但所获得的结果表明，以E2预处理后ChAT活性增加为指标的斜角带胆碱能功能的净增加，可能有助于激素诱导脊柱前凸和/或促黄体生成素激增的能力。
