Wang Fanfan, Tang Tao, Mao Ting, Duan Yuanyuan, Guo Xiaoliang, You Jingmao
Key Laboratory of Biology and Cultivation of Chinese Herbal Medicines, Ministry of Agriculture and Rural Affairs, Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi, China.
Hubei Engineering Research Center of Under-forest Economy, Hubei Academy of Agricultural Sciences, Wuhan, China.
Front Microbiol. 2023 May 30;14:1152865. doi: 10.3389/fmicb.2023.1152865. eCollection 2023.
. is a globally dispersed pathogenic fungus that causes southern blight disease in many crops and Chinese herbal medicine. The high degree of variation and diversity in the fungi altered population genetic structure. Therefore, the important factors of variation within the pathogen population should be considered during the development of management strategies for the disease.
In this study, isolates from 13 hosts in 7 provinces of China were collected and analyzed to identify their morphological features and perform molecular characterization. To develop EST-SSR primers, transcriptome sequencing was performed on isolated CB1, and its SSR loci were comprehensively analyzed. In addition, we analyzed the polymorphisms among different populations based on screened EST-SSR primers.
The results showed that all of these clean reads with total 36,165,475 assembled bases were clustered into 28,158 unigenes, ranged from 201 bp to 16,402 bp on the length, of which the average length was 1,284 bp. Of these, the SSR sequence appeared at an average interval of 15.43 kB, and the frequency of SSR was 0.0648 SSR/kB. Polymorphism of 9 primers was observed among 22 populations, and was verified by the Shannon's index (average = 1.414) and polymorphic information index (> 0.50). The genetic diversity analysis revealed diversity in all host populations and geographical populations. Further, molecular variance analysis (AMOVA) showed that the differences between groups were mainly related to geographical location. Based on cluster analysis, the 7 populations were roughly divided into 3 groups, and the results were highly consistent with those based on the geographical location, ultimately aligning with the results of STRUCTURE analysis.
The findings build on current knowledge of the distribution of in the southwest area of China, adding value to current knowledge base on the population structure and genetic diversity of , specifically in the context of Chinese herbal medicine cultivation in China. Overall, our findings may provide valuable information for breeding of crops with enhanced resistance toward .
……是一种全球分布的致病真菌，可在许多农作物和中药材中引发白绢病。真菌中高度的变异和多样性改变了群体遗传结构。因此，在制定该病害的管理策略时，应考虑病原菌群体内变异的重要因素。
在本研究中，收集并分析了来自中国7个省份13种寄主的……分离株，以鉴定其形态特征并进行分子表征。为开发EST-SSR引物，对分离得到的CB1进行转录组测序，并对其SSR位点进行综合分析。此外，基于筛选出的EST-SSR引物，分析了不同群体间的多态性。
结果表明，所有这些总共有36,165,475个组装碱基的 clean reads 被聚类成28,158个单基因，长度从201 bp到16,402 bp不等，平均长度为1,284 bp。其中，SSR序列平均间隔15.43 kB出现，SSR频率为0.0648 SSR/kB。在22个群体中观察到9种引物的多态性，并通过香农指数（平均值 = 1.414）和多态信息指数（> 0.50）进行了验证。遗传多样性分析揭示了所有寄主群体和地理群体中的多样性。此外，分子方差分析（AMOVA）表明，组间差异主要与地理位置有关。基于聚类分析，7个群体大致分为3组，结果与基于地理位置的结果高度一致，最终与STRUCTURE分析结果相符。
这些发现基于目前对……在中国西南地区分布的认识，为当前关于……群体结构和遗传多样性的知识库增添了价值，特别是在中国中药材种植的背景下。总体而言，我们的发现可能为培育对白绢病具有更强抗性的作物提供有价值的信息。
