增强生物传感的四倍放大和自供电能力：MoS@C 空心纳米棒介导的 DNA 六面体框架结构用于检测纳摩尔级肝癌肿瘤标志物。
Enhancing biosensing with fourfold amplification and self-powering capabilities: MoS@C hollow nanorods-mediated DNA hexahedral framework architecture for amol-level liver cancer tumor marker detection.
机构信息
School of Chemistry and Chemical Engineering, Guangxi Minzu University, Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products, Key Laboratory of Applied Analytical Chemistry (Guangxi Minzu University), Education Department of Guangxi Zhuang Autonomous Region, Nanning, 530006, China.
School of Chemistry and Chemical Engineering, Guangxi Minzu University, Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products, Key Laboratory of Applied Analytical Chemistry (Guangxi Minzu University), Education Department of Guangxi Zhuang Autonomous Region, Nanning, 530006, China.
出版信息
Anal Chim Acta. 2023 Aug 29;1271:341413. doi: 10.1016/j.aca.2023.341413. Epub 2023 May 23.
Two-dimensional carbon-coated molybdenum disulfide (MoS@C) hollow nanorods are combined with nucleic acid signal amplification strategies and DNA hexahedral nanoframework to construct a novel self-powered biosensing platform for ultra-sensitive dual-mode detection of tumor suppressor microRNA-199a. The nanomaterial is applied on carbon cloth and then modified with glucose oxidase or using as bioanode. A large number of double helix DNA chains are produced on bicathode by nucleic acid technologies including 3D DNA walker, hybrid chain reaction and DNA hexahedral nanoframework to adsorb methylene blue, producing high E signal. Methylene blue also is reduced and an increased RGB Blue value is observed. For microRNA-199a detection, the assay shows a extensive linear range of 0.0001-100 pM with a low detection limit of 4.94 amol/L (S/N = 3). The method has been applied to the detection of actual serum samples, providing a novel method for the accurate and sensitive detection of tumor markers.
二维碳包覆二硫化钼 (MoS@C) 空心纳米棒与核酸信号放大策略和 DNA 六方纳米框架相结合，构建了一种用于超灵敏双模式检测肿瘤抑制 microRNA-199a 的新型自供电生物传感平台。该纳米材料被应用于碳布上，并通过包括 3D DNA walker、杂交链式反应和 DNA 六方纳米框架在内的核酸技术进行葡萄糖氧化酶的修饰或作为生物阳极。大量的双链 DNA 链在双阴极上产生，通过核酸技术产生高 E 信号，包括 3D DNA walker、杂交链式反应和 DNA 六方纳米框架，用于吸附亚甲基蓝。亚甲基蓝也被还原，观察到 RGB Blue 值增加。对于 microRNA-199a 的检测，该分析方法显示出广泛的线性范围为 0.0001-100 pM，检测限低至 4.94 amol/L (S/N = 3)。该方法已应用于实际血清样本的检测，为肿瘤标志物的准确灵敏检测提供了新方法。
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