微甘菊提取物通过激活 FAK/Akt/mTOR 细胞信号通路增强皮肤伤口愈合活性。
Mikania micrantha extract enhances cutaneous wound healing activity through the activation of FAK/Akt/mTOR cell signaling pathway.
机构信息
Department of Veterinary Medicine, College of Veterinary Sciences & Animal Husbandry, Central Agricultural University, Jalukie, Imphal, Nagaland 797110, India.
Laboratory Animal Division, CSIR-Central Drug Research Institute, Lucknow, India.
出版信息
Injury. 2023 Aug;54(8):110856. doi: 10.1016/j.injury.2023.110856. Epub 2023 Jun 7.
Mikania micrantha (MM) has been traditionally used for various health benefits, including mental health, anti-inflammatory, wound dressing, and healing of sores. However, the molecular mechanisms and dose required for the wound healing activity of MM have yet to be reported. Therefore, a study was conducted to evaluate the wound healing potential of a cold methanolic extract of MM through in vitro and in vivo studies. Human dermal fibroblast adult (HDFa) cells were treated with 0 (control), 75 ng/ml, 125 ng/ml, 250 ng/ml, and 500 ng/ml of MMmethanolic extract (MME) for 24 h. MME at 75 ng/ml has significantly (p˂0.05) promoted HDFa cell proliferation and migration. Further, MME has also been shown to enhance the invasiveness of human umbilical vascular endothelial cells (HUVECs), indicating the neovasculature for wound healing. The tube formation assay demonstrated a significant (p<0.05) increase in the angiogenic effect of the MME starting at a concentration of 75 ng/ml as compared to the control. Treatment of excision wounds in Wistar rats with 5% and 10% MME ointment significantly enhanced wound contraction compared to control animals. Incision wounds in rats treated with 5% and 10% MME showed a significant (p<0.01) increase in tensile strength compared to control. HDFa cells, and granulation tissue collected on day 14 post-wounding, revealed the modulation of the FAK/Akt/mTOR cell signaling pathway during the enhancement of wound healing. The results of gel zymography showed increased activity of MMP-2 and MMP-9 in the HDFa cells after treatment with the extract. It is concluded that MMEcan potentially accelerate cutaneous wound healing.
薇甘菊（MM）传统上被用于各种健康益处，包括心理健康、抗炎、伤口敷料和溃疡愈合。然而，MM 促进伤口愈合的分子机制和所需剂量尚未报道。因此，进行了一项研究，通过体外和体内研究评估 MM 的冷甲醇提取物的伤口愈合潜力。将人真皮成纤维细胞（HDFa）用 0（对照）、75ng/ml、125ng/ml、250ng/ml 和 500ng/ml 的 MM 甲醇提取物（MME）处理 24h。75ng/ml 的 MME 显著（p<0.05）促进 HDFa 细胞增殖和迁移。此外，MME 还增强了人脐静脉内皮细胞（HUVEC）的侵袭性，表明为伤口愈合提供新的血管。管形成试验表明，与对照相比，75ng/ml 浓度的 MME 开始时显著（p<0.05）增加了血管生成作用。用 5%和 10% MME 软膏治疗 Wistar 大鼠的切除伤口，与对照动物相比，显著增强了伤口收缩。用 5%和 10% MME 治疗的大鼠切口伤口的拉伸强度与对照相比显著增加（p<0.01）。HDFa 细胞和伤后第 14 天收集的肉芽组织揭示了在增强伤口愈合过程中 FAK/Akt/mTOR 细胞信号通路的调节。凝胶酶谱法的结果表明，在用提取物处理后 HDFa 细胞中 MMP-2 和 MMP-9 的活性增加。因此，MME 可能具有加速皮肤伤口愈合的潜力。
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