CSIR-Institute of Himalayan Bioresource Technology, Palampur, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
CSIR-Institute of Himalayan Bioresource Technology, Palampur, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
Food Chem. 2023 Nov 15;426:136571. doi: 10.1016/j.foodchem.2023.136571. Epub 2023 Jun 9.
The impact of intermolecular copigmentation between five phenolic acids, two flavonoid and three amino acids with R. arboreum anthocyanins (ANS) and its isolated cyanidin-3-O-monoglycosides were investigated through experimental and theoretical approach. On addition of different copigments, phenolic acid induced strong hyperchromic (0.26-0.55 nm) and bathochromic shift (6.6-14.2 nm). The color intensity and stability of ANS with, storage at 4 °C & 25 °C, sunlight, oxidation and heat were evaluated by chromaticity, anthocyanin content, kinetic and structural simulation analysis. The strongest copigmentation reaction was observed with narningin (NA) and also showed high thermostability and highest half-life i.e. 3.39 h-1.24 h at 90-160 °C. The cyanidin-3-O-monoglycosides were analysed for their copigmentation effect and observations revealed that NA displayed best copigmentation effect to cyanidin-3-O-arabinoside (B) followed by cyanidin-3-O-galactoside (A), and cyanidin-3-O-rhamnoside (C). Additionally, structural simulation and steered molecular dynamics insights NA is the most favourable co-pigment involving π-π stacking and H-bonding.
通过实验和理论方法研究了五种酚酸、两种类黄酮和三种氨基酸与 R. arboreum 花色苷（ANS）及其分离的矢车菊素-3-O-单糖苷之间的分子间共色作用的影响。加入不同的共色剂后，酚酸诱导强增色（0.26-0.55nm）和红移（6.6-14.2nm）。通过色度、花青素含量、动力学和结构模拟分析，评估了 ANS 与、在 4°C 和 25°C 下储存、阳光、氧化和热的颜色强度和稳定性。与 narningin (NA) 的共色反应最强，在 90-160°C 下也表现出高热稳定性和最高半衰期，即 3.39h-1.24h。对氰花苷-3-O-单糖苷进行了共色作用分析，观察结果表明，NA 对氰花苷-3-O-阿拉伯糖苷（B）表现出最佳的共色作用，其次是氰花苷-3-O-半乳糖苷（A）和氰花苷-3-O-鼠李糖苷（C）。此外，结构模拟和导向分子动力学的见解表明，NA 是最有利的共色物质，涉及π-π堆积和氢键。
