Universidade Federal da Grande Dourados, Faculdade de Ciências Agrárias, Programa de Pós-graduação em Agronomia, Rodovia Dourados/Itahum, Km 12, 79.804-970, Dourados, MS, Brazil.
Universidade Federal da Grande Dourados, Faculdade de Ciências Biológicas e Ambientais, Programa de Pós-Graduação em Entomologia e Conservação da Biodiversidade, Rodovia Dourados/Itahum, Km 12, 79.804-970, Dourados, MS, Brazil.
Bull Entomol Res. 2023 Aug;113(4):546-554. doi: 10.1017/S0007485323000226. Epub 2023 Jun 19.
(Germar) (Coleoptera: Chrysomelidae) is a major pest of common bean ( L.; Fabales: Fabaceae), and adults can defoliate plants during the whole crop cycle. This study was conducted to evaluate the resistance to in 16 common bean genotypes (14 landraces and 2 cultivars), through three different experiments. In the laboratory, choice and no-choice feeding tests were performed to evaluate the percentage of leaf consumption. In the greenhouse, plant height, numbers of leaves, percentage of injured leaves, percentage of injury per leaf, weight of seeds, and survival were evaluated. Furthermore, trichome density, levels of peroxidase (POD), superoxide dismutase (SOD), and protein content in common bean leaves were assessed. In the laboratory, the genotypes Chumbinho Branco, Dobalde, Manteigado, IPR Tuiuiú, and 90D Mouro were the least consumed by . In the greenhouse, the genotypes Dobalde, Manteigado, and IPR Tuiuiú expressed tolerance to the pest, which was associated with a higher plant height and/or unchanged POD and SOD levels and protein content following insect feeding, and no reduction in seed production. The landrace 90D Mouro exhibited antixenosis and tolerance to , observed as a lower leaf injury, higher trichome density, lower protein contents, higher SOD level and no reduction in seed weight. Overall, we have shown that antixenosis and tolerance can help overcome damages resulting from feeding, with emphasis on four common bean genotypes that may be useful in plant breeding programs aimed at controlling in common bean crops.
（Coleoptera: Chrysomelidae）是普通豆（L.；Fabales: Fabaceae）的主要害虫，成虫在整个作物周期内可使植物落叶。本研究通过三个不同的实验评估了 16 个普通豆基因型（14 个地方品种和 2 个栽培品种）对 的抗性。在实验室中，进行了选择和非选择喂养测试，以评估叶片消耗的百分比。在温室中，评估了株高、叶片数、受伤叶片的百分比、每叶片受伤的百分比、种子重量和 的存活率。此外，还评估了普通豆叶片上的毛密度、过氧化物酶（POD）、超氧化物歧化酶（SOD）和蛋白质含量的水平。在实验室中，Chumbinho Branco、Dobalde、Manteigado、IPR Tuiuiú 和 90D Mouro 等基因型的 消耗最少。在温室中，Dobalde、Manteigado 和 IPR Tuiuiú 对 表现出耐受性，这与昆虫取食后植物高度增加和/或 POD 和 SOD 水平以及蛋白质含量不变，以及种子产量没有减少有关。地方品种 90D Mouro 表现出抗生性和对 的耐受性，表现为叶片损伤较低、毛密度较高、蛋白质含量较低、SOD 水平较高且种子重量没有减少。总体而言，我们已经表明，抗生性和耐受性有助于克服 取食造成的损害，重点关注四个普通豆基因型，它们可能对控制普通豆作物中的 有用植物育种计划。
