• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

组织损伤和出血性休克的组学特征在猪。

Omics Signatures of Tissue Injury and Hemorrhagic Shock in Swine.

机构信息

Department of Biochemistry and Molecular Genetics, University of Colorado Denver-Anschutz Medical Campus, Aurora, CO.

Department of Surgery, University of Colorado-Anschutz Medical Campus, Aurora, CO.

出版信息

Ann Surg. 2023 Dec 1;278(6):e1299-e1312. doi: 10.1097/SLA.0000000000005944. Epub 2023 Jun 19.

DOI:10.1097/SLA.0000000000005944
PMID:37334680
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10728352/
Abstract

OBJECTIVE

Advanced mass spectrometry methods were leveraged to analyze both proteomics and metabolomics signatures in plasma upon controlled tissue injury (TI) and hemorrhagic shock (HS)-isolated or combined-in a swine model, followed by correlation to viscoelastic measurements of coagulopathy via thrombelastography.

BACKGROUND

TI and HS cause distinct molecular changes in plasma in both animal models and trauma patients. However, the contribution to coagulopathy of trauma, the leading cause of preventable mortality in this patient population remains unclear. The recent development of a swine model for isolated or combined TI+HS facilitated the current study.

METHODS

Male swine (n=17) were randomized to either isolated or combined TI and HS. Coagulation status was analyzed by thrombelastography during the monitored time course. The plasma fractions of the blood draws (at baseline; end of shock; and at 30 minutes, 1, 2, and 4 hours after shock) were analyzed by mass spectrometry-based proteomics and metabolomics workflows.

RESULTS

HS-isolated or combined with TI-caused the most severe omic alterations during the monitored time course. While isolated TI delayed the activation of coagulation cascades. Correlation to thrombelastography parameters of clot strength (maximum amplitude) and breakdown (LY30) revealed signatures of coagulopathy which were supported by analysis of gene ontology-enriched biological pathways.

CONCLUSION

The current study provides a comprehensive characterization of proteomic and metabolomic alterations to combined or isolated TI and HS in a swine model and identifies early and late omics correlates to viscoelastic measurements in this system.

摘要

目的

利用先进的质谱方法分析受控组织损伤（TI）和失血性休克（HS）单独或联合作用于猪模型后的蛋白质组学和代谢组学特征，然后通过血栓弹性描记术与凝血功能的粘弹性测量相关联。

背景

TI 和 HS 在动物模型和创伤患者的血浆中引起不同的分子变化。然而，创伤导致的凝血功能障碍对该患者群体中导致可预防死亡率的主要原因的贡献尚不清楚。最近开发的单独或联合 TI+HS 的猪模型促成了本研究。

方法

雄性猪（n=17）随机分为单独或联合 TI 和 HS 组。在监测的时间过程中通过血栓弹性描记术分析凝血状态。在采血的血浆部分（基线；休克结束时；休克后 30 分钟、1 小时、2 小时和 4 小时）通过基于质谱的蛋白质组学和代谢组学工作流程进行分析。

结果

HS 单独或与 TI 联合导致监测时间过程中最严重的组学改变。而单独的 TI 延迟了凝血级联的激活。与血栓弹性图参数（最大振幅）和分解（LY30）的相关性显示了凝血功能障碍的特征，这些特征得到了对富集生物学途径的基因本体分析的支持。

结论

本研究提供了一个综合的猪模型中 TI 和 HS 单独或联合的蛋白质组学和代谢组学改变的描述，并确定了早期和晚期组学与该系统粘弹性测量的相关性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d15/10728352/a260bab8494b/nihms-1907428-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d15/10728352/61b90816be28/nihms-1907428-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d15/10728352/c6a5462888e3/nihms-1907428-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d15/10728352/a170c39308cf/nihms-1907428-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d15/10728352/d75a516cd74b/nihms-1907428-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d15/10728352/95e08df15037/nihms-1907428-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d15/10728352/c5d148483703/nihms-1907428-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d15/10728352/a260bab8494b/nihms-1907428-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d15/10728352/61b90816be28/nihms-1907428-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d15/10728352/c6a5462888e3/nihms-1907428-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d15/10728352/a170c39308cf/nihms-1907428-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d15/10728352/d75a516cd74b/nihms-1907428-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d15/10728352/95e08df15037/nihms-1907428-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d15/10728352/c5d148483703/nihms-1907428-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d15/10728352/a260bab8494b/nihms-1907428-f0007.jpg

相似文献

1
Omics Signatures of Tissue Injury and Hemorrhagic Shock in Swine.组织损伤和出血性休克的组学特征在猪。
Ann Surg. 2023 Dec 1;278(6):e1299-e1312. doi: 10.1097/SLA.0000000000005944. Epub 2023 Jun 19.
2
Hemorrhagic shock and tissue injury provoke distinct components of trauma-induced coagulopathy in a swine model.失血性休克和组织损伤在猪模型中引发了创伤性凝血病的不同成分。
Eur J Trauma Emerg Surg. 2023 Apr;49(2):1079-1089. doi: 10.1007/s00068-022-02148-x. Epub 2022 Nov 1.
3
FFP maintains normal coagulation while Kcentra induces a hypercoagulable state in a porcine model of pulmonary contusion and hemorrhagic shock.FFP 在维持正常凝血的同时，Kcentra 在猪肺挫伤和失血性休克模型中诱导出一种高凝状态。
J Trauma Acute Care Surg. 2022 Jul 1;93(1):124-129. doi: 10.1097/TA.0000000000003584. Epub 2022 Mar 7.
4
Thrombelastography indicates limitations of animal models of trauma-induced coagulopathy.血栓弹力图显示了创伤性凝血病动物模型的局限性。
J Surg Res. 2017 Sep;217:207-212. doi: 10.1016/j.jss.2017.05.027. Epub 2017 May 11.
5
Thrombelastography is better than PT, aPTT, and activated clotting time in detecting clinically relevant clotting abnormalities after hypothermia, hemorrhagic shock and resuscitation in pigs.在检测猪体温过低、失血性休克及复苏后临床上相关的凝血异常方面，血栓弹力图比凝血酶原时间（PT）、活化部分凝血活酶时间（aPTT）及活化凝血时间更具优势。
J Trauma. 2008 Sep;65(3):535-43. doi: 10.1097/TA.0b013e31818379a6.
6
Differential contributions of platelets and fibrinogen to early coagulopathy in a rat model of hemorrhagic shock.血小板和纤维蛋白原对失血性休克大鼠模型早期凝血病的不同作用
Thromb Res. 2016 May;141:58-65. doi: 10.1016/j.thromres.2016.03.007. Epub 2016 Mar 7.
7
Study on coagulation profiles and platelet function in trauma-induced coagulopathy caused by three types of injury.三种损伤致创伤性凝血病的凝血谱及血小板功能研究。
Injury. 2020 Jun;51(6):1312-1320. doi: 10.1016/j.injury.2020.02.081. Epub 2020 Feb 18.
8
Critical traumatic brain injury is associated with worse coagulopathy.严重创伤性脑损伤与更严重的凝血功能障碍有关。
J Trauma Acute Care Surg. 2021 Aug 1;91(2):331-335. doi: 10.1097/TA.0000000000003253.
9
Acute coagulopathy in a porcine venous hemorrhage and ischemia reperfusion model.猪静脉出血及再灌注模型中的急性凝血功能障碍。
Am J Surg. 2014 May;207(5):637-41; discussion 641. doi: 10.1016/j.amjsurg.2013.12.008. Epub 2014 Jan 31.
10
A combat casualty relevant dismounted complex blast injury model in swine.猪的与下车相关的复杂爆炸伤战斗伤员模型。
J Trauma Acute Care Surg. 2022 Aug 1;93(2S Suppl 1):S110-S118. doi: 10.1097/TA.0000000000003674. Epub 2022 May 12.

引用本文的文献

1
Increase in plasma succinate is associated with aerobic lactate production in a model of endotoxic shock.在内毒素休克模型中，血浆琥珀酸水平升高与有氧乳酸生成有关。
Exp Physiol. 2025 Apr;110(4):550-560. doi: 10.1113/EP092109. Epub 2025 Mar 19.
2
Navigating Hemorrhagic Shock: Biomarkers, Therapies, and Challenges in Clinical Care.应对失血性休克：生物标志物、治疗方法及临床护理中的挑战
Biomedicines. 2024 Dec 17;12(12):2864. doi: 10.3390/biomedicines12122864.

本文引用的文献

1
Red Blood Cell Omics and Machine Learning in Transfusion Medicine: Singularity Is Near.输血医学中的红细胞组学与机器学习：奇点将至。
Transfus Med Hemother. 2023 Mar 8;50(3):174-183. doi: 10.1159/000529744. eCollection 2023 Jun.
2
In vivo evaluation of the effect of sickle cell hemoglobin S, C and therapeutic transfusion on erythrocyte metabolism and cardiorenal dysfunction.体内评估镰状细胞血红蛋白 S、C 及治疗性输血对红细胞代谢和心肾功能障碍的影响。
Am J Hematol. 2023 Jul;98(7):1017-1028. doi: 10.1002/ajh.26923. Epub 2023 Apr 3.
3
Coagulation potential and the integrated omics of extracellular vesicles from COVID-19 positive patient plasma.
来自 COVID-19 阳性患者血浆的凝血潜能和细胞外囊泡的综合组学。
Sci Rep. 2022 Dec 23;12(1):22191. doi: 10.1038/s41598-022-26473-8.
4
Omics Markers of Red Blood Cell Transfusion in Trauma.创伤患者红细胞输血的组学标志物。
Int J Mol Sci. 2022 Nov 10;23(22):13815. doi: 10.3390/ijms232213815.
5
A pilot study of the metabolic profiles of apheresis platelets modified by donor age and sex and in vitro short-term incubation with sex hormones.供者年龄和性别对血小板代谢谱的影响及体外短期孵育性激素对血小板代谢谱的影响的初步研究。
Transfusion. 2022 Dec;62(12):2596-2608. doi: 10.1111/trf.17165. Epub 2022 Oct 30.
6
Hormones, age, and sex affect platelet responsiveness in vitro.激素、年龄和性别会影响体外血小板反应性。
Transfusion. 2022 Sep;62(9):1882-1893. doi: 10.1111/trf.17054. Epub 2022 Aug 5.
7
Recent Developments in Mouse Trauma Research Models: A Mini-Review.小鼠创伤研究模型的最新进展：一篇综述短文
Front Physiol. 2022 Apr 29;13:866617. doi: 10.3389/fphys.2022.866617. eCollection 2022.
8
Renal Microcirculation and Function in a Pig Model of Hemorrhagic Shock Resuscitation with Norepinephrine.去甲肾上腺素复苏失血性休克猪模型的肾微循环和功能
Am J Respir Crit Care Med. 2022 Jul 1;206(1):34-43. doi: 10.1164/rccm.202109-2120OC.
9
Erythrocyte transglutaminase-2 combats hypoxia and chronic kidney disease by promoting oxygen delivery and carnitine homeostasis.红细胞转谷氨酰胺酶-2 通过促进氧输送和肉碱稳态来对抗缺氧和慢性肾病。
Cell Metab. 2022 Feb 1;34(2):299-316.e6. doi: 10.1016/j.cmet.2021.12.019.
10
The Impact of Age and BMI on the VWF/ADAMTS13 Axis and Simultaneous Thrombin and Plasmin Generation in Hospitalized COVID-19 Patients.年龄和体重指数对住院COVID-19患者血管性血友病因子/ADAMTS13轴以及凝血酶和纤溶酶同时生成的影响
Front Med (Lausanne). 2022 Jan 10;8:817305. doi: 10.3389/fmed.2021.817305. eCollection 2021.