Wushu College, Henan University, Kaifeng, China.
School of Physical Education and Sport, Henan University, Kaifeng, China.
DNA Cell Biol. 2023 Aug;42(8):498-506. doi: 10.1089/dna.2022.0285. Epub 2023 Jul 28.
The purpose of this study was to find hub genes that may play key roles in skeletal muscle injury induced by jumping load. Twelve female Sprague Dawley rats were divided into the normal control (NC) group and the jumping-induced muscle injury (JI) group. After 6 weeks of jumping, transmission electron microscopy, hematoxylin-eosin staining, transcriptomics sequencing and genes analysis, interaction network prediction of multiple proteins, real-time PCR detection, and Western blotting were performed on gastrocnemius muscles from NC and JI groups. As compared with NC rats, excessive jumping can result in notable structural damage and inflammatory infiltration in JI rats. A total of 112 differentially expressed genes were confirmed in NC rats versus JI rats, with 59 genes upregulated and 53 genes downregulated. Using the online String database, four hub genes in the transcriptional regulatory network were targeted, including , , , and . All expression levels of , , , and mRNAs were decreased in JI rats compared with NC rats ( < 0.05 or < 0.01). All expression levels of c-Fos, EGR1, ATF3, and NOR1 proteins were upregulated in JI rats ( < 0.01, < 0.05, 0.05, < 0.01, respectively). Collectively, these findings indicate that , , , and genes may be functionally important in jumping-induced muscle injury.
本研究旨在寻找可能在跳跃负荷引起的骨骼肌损伤中发挥关键作用的枢纽基因。将 12 只雌性 Sprague Dawley 大鼠分为正常对照组（NC）和跳跃诱导肌肉损伤组（JI）。跳跃 6 周后，对 NC 组和 JI 组的比目鱼肌进行透射电镜、苏木精-伊红染色、转录组测序和基因分析、多种蛋白质的互作网络预测、实时 PCR 检测和 Western blot 分析。与 NC 大鼠相比，过度跳跃可导致 JI 大鼠出现明显的结构损伤和炎症浸润。NC 大鼠与 JI 大鼠相比，共鉴定出 112 个差异表达基因，其中 59 个基因上调，53 个基因下调。利用在线 String 数据库，针对转录调控网络中的 4 个枢纽基因，包括、、、。与 NC 大鼠相比，JI 大鼠中、、和的 mRNA 表达水平均降低（<0.05 或 <0.01）。JI 大鼠中 c-Fos、EGR1、ATF3 和 NOR1 蛋白的表达水平均上调（<0.01、<0.05、0.05、<0.01，分别）。综上，这些结果表明，、、和基因可能在跳跃诱导的肌肉损伤中具有重要的功能。
