Kaishev V G, Oleinik S A, Sycheva O V
Stavropol State Agrarian University, 355017, Stavropol, Russian Federation.
Vopr Pitan. 2023;92(2):109-115. doi: 10.33029/0042-8833-2023-92-2-109-115. Epub 2023 Mar 1.
Polyunsaturated fatty acids (PUFA) consumed with dietary fats are physiologically active substances involved in metabolic processes in the organism, in particular, carbohydrate-fat and cholesterol metabolism, regulation of redox processes. Butter is one of the main food items of the daily human diet. The basis of butter is milk fat, which is characterized by a rich fatty acid composition and unique properties. The possibility of obtaining butter with the desired ratio of saturated and unsaturated fatty acids without making adjustments to the technological process using milk with high PUFA content has been investigated. of the study was to obtain butter enriched with PUFA from the milk of Holstein cows with a modified fatty acid composition towards an increased content of unsaturated fatty acids. . For the research, dairy raw materials from lactating Holstein cows were used, the milking herd included 881 cows. Dairy raw materials were studied, selected individually from each cow. According to the results of an in-depth assessment of the composition of milk lipid fractions, a group of cows was isolated after the first calving - 15 heads, whose milk was characterized by an increased content of unsaturated fatty acids. The content of milk fat, protein, lactose and fatty acid composition of milk were determined by infrared spectrometry with Fourier transform. Butter was produced from the milk of the experimental and control (combined) groups, which was evaluated by organoleptic (on a 20-point scale), physico-chemical parameters, including fatty acid composition determined by gas chromatography. . Milk obtained from cows of the experimental group differed downward from the combined milk produced in the whole herd in terms of protein and casein content by 12.8%, dry matter - by 4.4%, SOMO - by 3.1%. It varied upwards in milk sugar level (by 3.2%). In the milk fat of the milk of the experimental group of cows, the total amount of saturated fatty acids was reduced by 14.9%, and unsaturated fatty acid level was increased by 12.6%; as a result, the ratio of unsaturated to saturated fatty acids was higher by 31.7%. The butter produced from the milk of cows of the experimental group was slightly inferior to the control sample in terms of the characteristics of «taste and smell», «consistency» and total score. At the same time, the prototype fat differed from the control one by a more pronounced, saturated yellow color. Evaluation of the fatty acid composition of the experimental and control fat samples showed the advantage of the prototype in terms of PUFA content compared to the control. The total amount of unsaturated fatty acids exceeded the same indicator for the control sample by 8.3%, while the superiority of polyunsaturated fatty acids was 14.4%. At the same time, saturated fatty acid level in the experimental sample was 4.2% less, but the amount of volatile low-molecular- weight fatty acids was significantly reduced compared to the control sample - by 19.1%. . The results obtained show the possibility and open the prospect of obtaining butter with a more favorable ratio of fatty acids in favor of PUFAs by selecting cows for milk with a higher ratio of unsaturated and saturated fatty acids and further selection aimed at an increased content of PUFAs in milk.
与膳食脂肪一起摄入的多不饱和脂肪酸(PUFA)是参与机体代谢过程的生理活性物质,特别是碳水化合物 - 脂肪和胆固醇代谢以及氧化还原过程的调节。黄油是人类日常饮食的主要食物之一。黄油的主要成分是乳脂肪,其特点是脂肪酸组成丰富且具有独特性质。研究了在不改变工艺过程的情况下,使用高PUFA含量的牛奶获得具有所需饱和脂肪酸与不饱和脂肪酸比例的黄油的可能性。该研究的目的是从荷斯坦奶牛的牛奶中获得富含PUFA的黄油,其脂肪酸组成经过改良,不饱和脂肪酸含量增加。在研究中,使用了来自泌乳荷斯坦奶牛的乳制品原料,挤奶牛群包括881头奶牛。对乳制品原料进行了研究,从每头奶牛中单独选取。根据对乳脂质部分组成的深入评估结果,在首次产犊后分离出一组奶牛——15头,其牛奶的特点是不饱和脂肪酸含量增加。通过傅里叶变换红外光谱法测定牛奶中的乳脂肪、蛋白质、乳糖和脂肪酸组成。用实验组和对照组(混合组)的牛奶制作黄油,并通过感官评价(20分制)、理化参数进行评估,包括通过气相色谱法测定的脂肪酸组成。实验组奶牛产的牛奶在蛋白质和酪蛋白含量方面比整个牛群产的混合牛奶低12.8%,干物质低4.4%,SOMO低3.1%。其乳糖水平有所上升(3.2%)。实验组奶牛牛奶中的乳脂肪中,饱和脂肪酸总量减少了14.9%,不饱和脂肪酸水平增加了12.6%;结果,不饱和脂肪酸与饱和脂肪酸的比例高出31.7%。实验组奶牛牛奶制作的黄油在“味道和气味”、“稠度”和总分方面略逊于对照样品。同时,原型脂肪与对照脂肪的区别在于颜色更明显、更饱和的黄色。对实验和对照脂肪样品的脂肪酸组成评估表明,与对照相比,原型在PUFA含量方面具有优势。不饱和脂肪酸总量比对照样品高出8.3%,而多不饱和脂肪酸的优势为14.4%。同时,实验样品中的饱和脂肪酸水平低4.2%,但与对照样品相比,挥发性低分子量脂肪酸的含量显著降低——降低了19.1%。所获得的结果表明,通过选择不饱和脂肪酸与饱和脂肪酸比例更高的奶牛产奶,并进一步选择以提高牛奶中PUFA的含量,有可能获得脂肪酸比例更有利于PUFA的黄油,并开辟了前景。