从一年生丰年虾胚胎中提取活原肠胚细胞的方案。
Protocol for extracting live blastoderm cells from embryos of annual killifish.
机构信息
Laboratory for Mechanobiology of Transforming Systems, Institute for Biological and Medical Engineering, Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 7820436, Chile; Laboratory for Molecular Mechanics of Cell Adhesion, Department of Physiology Pontificia Universidad Católica de Chile, Santiago 8320165, Chile.
Integrative Biology Program, Institute of Biomedical Sciences, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile; Biomedical Neuroscience Institute, Santiago 8380453, Chile.
出版信息
STAR Protoc. 2023 Sep 15;4(3):102344. doi: 10.1016/j.xpro.2023.102344. Epub 2023 Jun 22.
The implementation of in vitro approaches using undifferentiated embryonic cells from annual killifish to complement existing in vivo developmental studies has been hindered by a lack of efficient isolation techniques. Here, we present a protocol to isolate annual killifish blastoderm cells, at the epiboly and early dispersion phase, from embryos. We describe steps for hair removal, embryo cleaning, dechorionation, and cell purification. This protocol may also be used to develop strategies to isolate cells from embryos presenting similar challenges.
利用一年生食蚊鱼未分化的胚胎细胞进行体外方法的实施，以补充现有的体内发育研究，一直受到缺乏有效分离技术的阻碍。在这里，我们提出了一种从胚胎的胚盘和早期分散阶段分离一年生食蚊鱼胚泡细胞的方案。我们描述了去除毛发、胚胎清洗、去壳和细胞纯化的步骤。该方案也可用于开发从具有类似挑战的胚胎中分离细胞的策略。
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