Université de Montréal, Department of Biochemistry and Molecular Biology, Montréal, QC, H3T 1J4, Canada.
Curr Opin Struct Biol. 2023 Aug;81:102643. doi: 10.1016/j.sbi.2023.102643. Epub 2023 Jun 21.
PARP enzymes create ADP-ribose modifications to regulate multiple facets of human biology, and some prominent PARP family members are best known for the nucleic acid interactions that regulate their activities and functions. Recent structural studies have highlighted PARP interactions with nucleic acids, in particular for PARP enzymes that detect and respond to DNA strand break damage. These studies build on our understanding of how DNA break detection is linked to the catalysis of ADP-ribose modifications, provide insights into distinct modes of DNA interaction, and shed light on the mechanisms of PARP inhibitor action. PARP enzymes have several connections to RNA biology, including the detection of the genomes of RNA viruses, and recent structural work has highlighted how PARP13/ZAP specifically targets viral genomes enriched in CG dinucleotides.
PARP 酶通过产生 ADP-核糖修饰来调节人类生物学的多个方面，一些突出的 PARP 家族成员以调节其活性和功能的核酸相互作用而闻名。最近的结构研究强调了 PARP 与核酸的相互作用，特别是对于那些检测和响应 DNA 链断裂损伤的 PARP 酶。这些研究建立在我们对 DNA 断裂检测如何与 ADP-核糖修饰的催化相关联的理解之上，为不同的 DNA 相互作用模式提供了深入的见解，并阐明了 PARP 抑制剂作用的机制。PARP 酶与 RNA 生物学有几个联系，包括对 RNA 病毒基因组的检测，最近的结构研究强调了 PARP13/ZAP 如何特异性地靶向富含 CG 二核苷酸的病毒基因组。
