Validity of ActivPAL CREA software detection of sitting and lying during free-living conditions.

. Approaches to differentiate sitting and lying are available within the default activPAL software from a single thigh-worn monitor. Dual-monitor methods use multiple monitors positioned on the thigh and torso to characterize sitting versus lying. We evaluated the validity between these two methods to measure waking, sitting, and lying time in free-living conditions. We also examined if the degree-threshold distinguishing sitting/lying for the dual-monitor (<30° and <45°) impacted results.. Thirty-five young adults (24 ± 3 years, 16 females) wore an activPAL 24 h per day on their thigh and torso during free-living conditions (average: 6.8 ± 1.0 d, 239 total). Data were processed using the default activPAL software (thigh-only) or a custom MATLAB program (dual-monitor).. The single-monitor recorded less lying time (59 ± 99 min d) and more sitting time (514 ± 203 min d) than the dual-monitor method regardless of 30° (lying: 85 ± 94 min d; sitting: 488 ± 166 min d) or 45° lying threshold (lying: 170 ± 142, sitting: 403 ± 164 min d; all,< 0.001). The single monitor lying time was weakly correlated to the dual-monitor (30°:= 0.25, 45°:= 0.21; both,< 0.001), whereas sitting was moderate-strong (30°:= 0.76, 45°:= 0.58; both,< 0.001). However, the mean absolute error was 81 min d(30°) and 132 min d(45°) for both lying and sitting.. The method of differentiating sitting/lying from a single thigh-worn activPAL records more sitting time and less lying time compared to a dual-monitor configuration (regardless of degree-threshold) that considered the position of the torso. A further refinement of algorithms or implementation of multiple-monitor methods may be needed for researchers to derive detailed sedentary positions.