International Clinical Research Center, Department of Global Health, Schools of Medicine and Public Health, University of Washington, Seattle, Washington, USA.
Department of Epidemiology, School of Public Health, University of Washington, Seattle, Washington, USA.
J Infect Dis. 2023 Oct 18;228(8):1066-1070. doi: 10.1093/infdis/jiad231.
We evaluated the performance of rapid antigen (RAg) and antibody (RAb) microfluidic diagnostics with serial sampling of 71 participants at 6 visits over 2 months following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Rapid tests showed strong agreement with laboratory references (κAg = 81.0%; κAb = 87.8%). RAg showed substantial concordance to both virus growth in culture and PCR positivity 0-5 days since symptom onset (κAg-culture = 60.1% and κAg-PCR = 87.1%). PCR concordance to virus growth in culture was similar (κPCR-culture = 70.0%), although agreement between RAg and culture was better overall (κAg-culture = 45.5% vs κPCR-culture = 10.0%). Rapid antigen and antibody testing by microfluidic immunofluorescence platform are highly accurate for characterization of acute infection.
我们评估了快速抗原（RAg）和抗体（RAb）微流控诊断在 SARS-CoV-2 感染后 2 个月内 6 次随访中对 71 名参与者的连续采样的性能。快速检测与实验室参考具有很强的一致性（κAg = 81.0%；κAb = 87.8%）。RAg 与培养物中的病毒生长和症状出现后 0-5 天的 PCR 阳性具有实质性的一致性（κAg-培养物 = 60.1%和 κAg-PCR = 87.1%）。与培养物中的病毒生长相比，PCR 的一致性也相似（κPCR-培养物 = 70.0%），尽管 RAg 和培养物的总体一致性更好（κAg-培养物 = 45.5%，而 κPCR-培养物 = 10.0%）。通过微流控免疫荧光平台进行的快速抗原和抗体检测对于急性感染的特征具有高度准确性。
